NAME¶
bdgcmp - Calculate scores using certain method by comparing a bedGraph file from
treatment and a file from control representing local bias
SYNOPSIS¶
bdgcmp <-t TREATMENT.BEDGRAPH> <-c CONTROL.BEDGRAPH>
<-o OUTPUT.BEDGRAPH> [
-m METHOD]
DESCRIPTION¶
Calculate scores using certain method by comparing a bedGraph file from
treatment and a file from control representing local bias.
OPTIONS¶
- --version
- show program's version number and exit
- -h, --help
- Show this help message and exit.
- -t TFILE, --tfile=TFILE
- Required: Treatment bedGraph file, e.g. *_treat_pileup.bdg from
MACSv2
- -c CFILE, --cfile=CFILE
- Required: Control bedGraph file, e.g. *_control_lambda.bdg from
MACSv2
- -o OFILE, --output=OFILE
- Required: The output bedGraph file to write scores.
- -m METHOD, --method=METHOD
- Method to use while calculating a score in any bin by comparing treatment
value and control value. Available choices are: ppois, substract, divide
which represent Poisson Pvalue ( -log10(pvalue) form) using control
as lambda and treatment as observation, substraction from treatment, fold
change which may be problematic if there are zero in control. Default
option is ppois.