.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.40.13. .TH BDGCMP "1" "December 2012" "bdgcmp 2.0.9.1" "User Commands" .SH NAME bdgcmp \- Calculate scores using certain method by comparing a bedGraph file from treatment and a file from control representing local bias .SH SYNOPSIS .B bdgcmp \fI<-t TREATMENT.BEDGRAPH> <-c CONTROL.BEDGRAPH> <-o OUTPUT.BEDGRAPH> \fR[\fI-m METHOD\fR] .SH DESCRIPTION Calculate scores using certain method by comparing a bedGraph file from treatment and a file from control representing local bias. .SH OPTIONS .TP \fB\-\-version\fR show program's version number and exit .TP \fB\-h\fR, \fB\-\-help\fR Show this help message and exit. .TP \fB\-t\fR TFILE, \fB\-\-tfile\fR=\fITFILE\fR Required: Treatment bedGraph file, e.g. *_treat_pileup.bdg from MACSv2 .TP \fB\-c\fR CFILE, \fB\-\-cfile\fR=\fICFILE\fR Required: Control bedGraph file, e.g. *_control_lambda.bdg from MACSv2 .TP \fB\-o\fR OFILE, \fB\-\-output\fR=\fIOFILE\fR Required: The output bedGraph file to write scores. .TP \fB\-m\fR METHOD, \fB\-\-method\fR=\fIMETHOD\fR Method to use while calculating a score in any bin by comparing treatment value and control value. Available choices are: ppois, substract, divide which represent Poisson Pvalue (\fB\-log10\fR(pvalue) form) using control as lambda and treatment as observation, substraction from treatment, fold change which may be problematic if there are zero in control. Default option is ppois.