|PRIMER_DESIGNER(1p)||User Contributed Perl Documentation||PRIMER_DESIGNER(1p)|
NAME¶primer_designer.pl -- command-line interface Bio::PrimerDesigner
./primer_designer.pl [options] [dna_sequence or file] Options: -p|--program Program (default "primer3") -n|--number Number of primer sets to return (default "5") -b|--binary Path to binary executable (default "/usr/local/bin") -m|--method "local" or "remote" (default "local") -u|--url URL of remote primer3/e-PCR system (default "aceserver.biotech.ubc.ca/cgi-bin/primer_designer.cgi") --list-aliases Print alias list to primer3 input options and exit --list-params Print a list of primer3/e-PCR input options and exit -h|--help Print help and exit
DESCRIPTION¶This script tests/demonstrates the Bio::PrimerDesigner interface to primer3 and e-PCR. It can be used with a local installation of the unix binaries or remotely via an HTTP/CGI request.
AUTHOR¶Copyright (C) 2003-2009 Sheldon McKay <firstname.lastname@example.org>, Ken Youens-Clark <email@example.com>.
LICENSE¶This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; version 3 or any later version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.
You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.