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PRIMER_DESIGNER(1p) User Contributed Perl Documentation PRIMER_DESIGNER(1p)

NAME -- command-line interface Bio::PrimerDesigner


    ./ [options] [dna_sequence or file]


  -p|--program   Program (default "primer3")
  -n|--number    Number of primer sets to return (default "5")
  -b|--binary    Path to binary executable (default "/usr/local/bin")
  -m|--method    "local" or "remote" (default "local")
  -u|--url       URL of remote primer3/e-PCR system (default 
  --list-aliases Print alias list to primer3 input options and exit
  --list-params  Print a list of primer3/e-PCR input options and exit
  -h|--help      Print help and exit


This script tests/demonstrates the Bio::PrimerDesigner interface to primer3 and e-PCR. It can be used with a local installation of the unix binaries or remotely via an HTTP/CGI request.


Copyright (C) 2003-2009 Sheldon McKay <>, Ken Youens-Clark <>.




This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; version 3 or any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.

2018-07-18 perl v5.26.2