NAME¶

SYNOPSIS¶

    $pathway = new Bio::Tools::PSort::Pathway('-path' => [ ... ]);

    @results = $pathway->traverse($seq);

DESCRIPTION¶

Since the Pathway modules interface for defining new trees is rather low level, it is recommended that end users use either of the Bio::Tools::PSort::Pathway::Perl or Bio::Tools::PSort::Pathway::XML modules to create a binary tree.

[1] As described in the paper 'Expert System for Predicting the Protein Localization Sites in Gram-Negative Bacteria' by Kenta Nakai and Minoru Kanehisa.

CONSTRUCTOR¶

    $pathway = new Bio::Tools::PSort::Pathway('-path' => [ ... ]);

The constructor for the Pathway object accepts a single named parameter, '-path', which is an arrayref containing a description of the pathway. Each node in the pathway is represented by an array ref containing 1 to 4 elements, which are in order, a reference to a Bio::Tools::PSort::ModuleI subclass, a reference to a subroutine, and two other node structures which will be executed if the subroutine returns either true or false values.

Upon arriving at a node in the tree, the module is first run on the sequence being analyzed, and it's results is passed to the subroutine. If the subroutine returns true, then the traversal continues with the third parameter, another tree node, otherwise traversal is sent down the fourth parameter, which is also another node in the tree.

For example:

    $mod1 = new Bio::Tools::PSort::Module::Regexp();
    $mod2 = new Bio::Tools::PSort::Module::SCLBLAST();
    $mod3 = new Bio::Tools::PSort::Module::SPaseI();

    $func = sub {
              my $res = shift;

              return ($res->score()->{cyoplasm} > 0.50);
            };

    $path = [$mod1, $func, [$mod2], [$mod3]];

    $pathway = new Bio::Tools::PSort::Pathway('-path' => $path);

This example describes a simple tree with three nodes. The first node consists of the Regular Expression module. If the result returned from the Regexp module has score higher that 0.50 for the cytoplasm localization, then the subroutine will return true, and control flow will be directed to the SCLBLAST module. If the score is less than or equal to 0.50, the control flow will be sent to the SPaseI module.

METHODS¶

    @results = $pathway->traverse($seq);

The traverse method is used to send a sequence down the pathway. An array of Bio::Tools::PSort::Score object is returned (each module returns a single Score object, they are collected as the pathway is traversed and returned as a group).

SEE ALSO¶

AUTHOR¶

 Fiona Brinkman, Cory Spencer <psort-list@sfu.ca>
 Brinkman Laboratory, Simon Fraser University, BC, CANADA

OVERVIEW¶

The standalone version of PSORT-B is distributed under the GNU General Public Licence (Gnu GPL) (see the LICENSE file included in the download) by the Brinkman Laboratory, Simon Fraser University, Burnaby, B.C., Canada.

This standalone version of PSORT-B has initially been developed for the Linux environment.

This document describes the installation of the PSORT-B version 1.1.4 command line program and the PSORT-B server packages. For most purposes, following the installation instructions for the command line version will be sufficient.

For further information, please contact psort-mail@sfu.ca.

LICENSE¶

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA