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TCODE(1e) EMBOSS Manual for Debian TCODE(1e)


tcode - Identify protein-coding regions using Fickett TESTCODE statistic


tcode -sequence seqall -datafile datafile -window integer -step integer -plot toggle -outfile report -graph xygraph

tcode -help


tcode is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is part of the "Nucleic:Gene finding" command group(s).


Input section

-sequence seqall

-datafile datafile

The default data file is Etcode.dat and contains coding probabilities for each base. The probabilities are for both positional and compositional information. Default value: Etcode.dat

Required section

-window integer

This is the number of nucleotide bases over which the TESTCODE statistic will be performed each time. The window will then slide along the sequence, covering the same number of bases each time. Default value: 200

Advanced section

-step integer

The selected window will, by default, slide along the nucleotide sequence by three bases at a time, retaining the frame (although the algorithm is not frame sensitive). This may be altered to increase or decrease the increment of the slide. Default value: 3

Output section

-plot toggle

On selection a graph of the sequence (X axis) plotted against the coding score (Y axis) will be displayed. Sequence above the green line is coding, that below the red line is non-coding. Default value: N

-outfile report

-graph xygraph


Bugs can be reported to the Debian Bug Tracking system (, or directly to the EMBOSS developers (


tcode is fully documented via the tfm(1) system.


Debian Med Packaging Team <>

Wrote the script used to autogenerate this manual page.


This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be redistributed under the same terms as EMBOSS itself.

05/11/2012 EMBOSS 6.4.0