.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.12. .TH YANAGIBA "1" "June 2020" "yanagiba 1.0.0" "User Commands" .SH NAME yanagiba \- Filters low quality Oxford Nanopore reads basecalled with Albacore .SH DESCRIPTION usage: yanagiba [\-h] \fB\-i\fR INFILE [\-s SUMMARYFILE] [\-o OUTFILE] [\-l MINLEN] .IP [\-q MINQUAL] [\-\-headtrim HEADTRIM] [\-\-tailtrim TAILTRIM] [\-u] .PP Filter and slice Nanopore reads which have been basecalled with Albacore. Takes fastq.gz and an Albacore summary file. .SS "optional arguments:" .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-i\fR INFILE, \fB\-\-infile\fR INFILE Input fastq.gz file. .TP \fB\-s\fR SUMMARYFILE, \fB\-\-summaryfile\fR SUMMARYFILE Albacore summary file with header row. .TP \fB\-o\fR OUTFILE, \fB\-\-outfile\fR OUTFILE Write filtered reads to this file in .bgz format. .TP \fB\-l\fR MINLEN, \fB\-\-minlen\fR MINLEN Exclude reads shorter than this length. Default: 0 .TP \fB\-q\fR MINQUAL, \fB\-\-minqual\fR MINQUAL Minimum quality score to retain a read. Default: 10 .TP \fB\-\-headtrim\fR HEADTRIM Trim x bases from beginning of each read. Default: 0 .TP \fB\-\-tailtrim\fR TAILTRIM Trim x bases from end of each read. Default: None .TP \fB\-u\fR, \fB\-\-forceunique\fR Enforce unique reads. Only store first instance of a read from fastq input where readID occurs multiple times. .SH AUTHOR This manpage was written by Nilesh Patra for the Debian distribution and can be used for any other usage of the program.