.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.49.2. .TH VG "1" "May 2022" "vg version v1.30.0 "Carentino"" "User Commands" .SH NAME vg \- tools for working with genome variation graphs .SH DESCRIPTION vg: variation graph tool, version v1.30.0 "Carentino" .PP usage: vg [options] .SS "main mapping and calling pipeline:" .TP \fB\-\-\fR construct graph construction .TP \fB\-\-\fR index index graphs or alignments for random access or mapping .TP \fB\-\-\fR map MEM\-based read alignment .TP \fB\-\-\fR giraffe fast haplotype\-aware short read alignment .TP \fB\-\-\fR augment augment a graph from an alignment .TP \fB\-\-\fR pack convert alignments to a compact coverage index .TP \fB\-\-\fR call call or genotype VCF variants .TP \fB\-\-\fR help show all subcommands .SS "useful graph tools:" .TP \fB\-\-\fR autoindex produce indexes for other subcommands .TP \fB\-\-\fR deconstruct create a VCF from variation in the graph .TP \fB\-\-\fR gbwt build and manipulate GBWTs .TP \fB\-\-\fR ids manipulate node ids .TP \fB\-\-\fR minimizer build a minimizer index or a syncmer index .TP \fB\-\-\fR mod filter, transform, and edit the graph .TP \fB\-\-\fR prune prune the graph for GCSA2 indexing .TP \fB\-\-\fR sim simulate reads from a graph .TP \fB\-\-\fR snarls compute snarls and their traversals .TP \fB\-\-\fR stats metrics describing graph and alignment properties .TP \fB\-\-\fR view format conversions for graphs and alignments .SS "specialized graph tools:" .TP \fB\-\-\fR add add variants from a VCF to a graph .TP \fB\-\-\fR align local alignment .TP \fB\-\-\fR annotate annotate alignments with graphs and graphs with alignments .TP \fB\-\-\fR chunk split graph or alignment into chunks .TP \fB\-\-\fR circularize circularize a path within a graph .TP \fB\-\-\fR concat concatenate graphs tail\-to\-head .TP \fB\-\-\fR convert convert graphs between handle\-graph compiant formats .TP \fB\-\-\fR depth estimate sequencing depth .TP \fB\-\-\fR dotplot generate the dotplot matrix from the embedded paths in an xg index .TP \fB\-\-\fR filter filter reads .TP \fB\-\-\fR gamcompare compare alignment positions .TP \fB\-\-\fR gampcompare compare multipath alignment positions .TP \fB\-\-\fR gamsort Sort a GAM file or index a sorted GAM file. .TP \fB\-\-\fR genotype Genotype (or type) graphs, GAMS, and VCFs. .TP \fB\-\-\fR inject lift over alignments for the graph .TP \fB\-\-\fR mpmap multipath alignments of reads to a graph .TP \fB\-\-\fR paths traverse paths in the graph .TP \fB\-\-\fR rna construct spliced variation graphs and transcript paths .TP \fB\-\-\fR simplify graph simplification .TP \fB\-\-\fR surject map alignments onto specific paths .TP \fB\-\-\fR trace trace haplotypes .TP \fB\-\-\fR vectorize transform alignments to simple ML\-compatible vectors .TP \fB\-\-\fR viz render visualizations of indexed graphs and read sets .SS "developer commands:" .TP \fB\-\-\fR benchmark run and report on performance benchmarks .TP \fB\-\-\fR cluster find and cluster mapping seeds .TP \fB\-\-\fR find use an index to find nodes, edges, kmers, paths, or positions .TP \fB\-\-\fR mcmc Finds haplotypes based on reads using MCMC methods .TP \fB\-\-\fR test run unit tests .TP \fB\-\-\fR validate validate the semantics of a graph or gam .TP \fB\-\-\fR version version information .PP For technical support, please visit: https://www.biostars.org/t/vg/ .PP Compiled with g++ (Debian 11.3.0\-1) 11.3.0 on Linux Linked against libstd++ 20220421 Built by buildd@x86\-csail\-01