.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.15. .TH TOMBO "1" "June 2020" "tombo 1.5.1" "User Commands" .SH NAME tombo \- identification of modified nucleotides from raw nanopore sequencing data .SH SYNOPSIS .B tombo [\-h] [\-v] {resquiggle,preprocess,filter,detect_modifications,text_output,build_model,plot} \&... .SH DESCRIPTION Tombo is a suite of tools primarily for the identification of modified nucleotides from nanopore sequencing data. .PP Tombo also provides tools for the analysis and visualization of raw nanopore signal. .SH "Tombo command groups (additional help available within each command group):" .TP resquiggle Re\-annotate raw signal with genomic alignment from existing basecalls. .TP preprocess Pre\-process nanopore reads for Tombo processing. .TP filter Apply filter to Tombo index file for specified criterion. .TP detect_modifications Perform statistical testing to detect non\-standard nucleotides. .TP text_output Output Tombo results in text files. .TP build_model Create canonical and alternative base Tombo models. .TP plot Save plots to visualize raw nanopore signal or testing results. .SS "optional arguments:" .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-v\fR, \fB\-\-version\fR show Tombo version and exit. .SH AUTHOR This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.