.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.49.2. .TH WEBLOGO "1" "November 2022" "WebLogo 3.7.12" "User Commands" .SH NAME WebLogo \- create sequence logos from biological sequence alignments .SH SYNOPSIS .B weblogo [\fI\,options\/\fR] \fI\,< sequence_data.fa > sequence_logo.eps\/\fR .SH DESCRIPTION Create sequence logos from biological sequence alignments. .SH OPTIONS .TP \fB\-\-version\fR show program's version number and exit .TP \fB\-h\fR \fB\-\-help\fR show this help message and exit .IP Input/Output Options: .TP \fB\-f\fR \fB\-\-fin\fR FILENAME Sequence input file (default: stdin) .TP \fB\-\-upload\fR URL Upload input file from URL .TP \fB\-D\fR \fB\-\-datatype\fR FORMAT Type of multiple sequence alignment or position weight matrix file: (clustal, fasta, msf, genbank, nbrf, nexus, phylip, stockholm, intelligenetics, table, array, transfac) .TP \fB\-o\fR \fB\-\-fout\fR FILENAME Output file (default: stdout) .TP \fB\-F\fR \fB\-\-format\fR FORMAT Format of output: eps (default), png, png_print, pdf, jpeg, svg, logodata .IP Logo Data Options: .TP \fB\-A\fR \fB\-\-sequence\-type\fR TYPE The type of sequence data: 'protein', 'rna' or \&'dna'. .TP \fB\-a\fR \fB\-\-alphabet\fR ALPHABET The set of symbols to count, e.g. 'AGTC'. All characters not in the alphabet are ignored. If neither the alphabet nor sequence\-type are specified then weblogo will examine the input data and make an educated guess. See also \fB\-\-sequence\-type\fR, \fB\-\-ignore\-lower\-case\fR .TP \fB\-U\fR \fB\-\-units\fR UNIT A unit of entropy ('bits' (default), 'nats', \&'digits'), or a unit offree energy ('kT', \&'kJ/mol', 'kcal/mol'), or 'probability' for probabilities .TP \fB\-\-composition\fR COMP. The expected composition of the sequences: \&'auto' (default), 'equiprobable', 'none' (do not perform any compositional adjustment), a CG percentage, a species name (e.g. 'E. coli', \&'H. sapiens'), or an explicit distribution (e.g. "{'A':10, 'C':40, 'G':40, 'T':10}"). The automatic option uses a typical distribution for proteins and equiprobable distribution for everything else. .TP \fB\-\-weight\fR NUMBER The weight of prior data. Default depends on alphabet length .TP \fB\-i\fR \fB\-\-first\-index\fR INDEX Index of first position in sequence data (default: 1) .TP \fB\-l\fR \fB\-\-lower\fR INDEX Lower bound of sequence to display .TP \fB\-u\fR \fB\-\-upper\fR INDEX Upper bound of sequence to display .IP Transformations: .IP Optional transformations of the sequence data. .TP \fB\-\-ignore\-lower\-case\fR Disregard lower case letters and only count upper case letters in sequences. .TP \fB\-\-reverse\fR reverse sequences .TP \fB\-\-complement\fR complement nucleic sequences .TP \fB\-\-revcomp\fR reverse complement nucleic sequences .IP Logo Format Options: .IP These options control the format and display of the logo. .TP \fB\-s\fR \fB\-\-size\fR LOGOSIZE Specify a standard logo size (small, medium (default), large) .TP \fB\-n\fR \fB\-\-stacks\-per\-line\fR COUNT Maximum number of logo stacks per logo line. (default: 40) .TP \fB\-t\fR \fB\-\-title\fR TEXT Logo title text. .TP \fB\-\-label\fR TEXT A figure label, e.g. '2a' .TP \fB\-X\fR \fB\-\-show\-xaxis\fR YES/NO Display sequence numbers along x\-axis? (default: True) .TP \fB\-x\fR \fB\-\-xlabel\fR TEXT X\-axis label .TP \fB\-\-annotate\fR TEXT A comma separated list of custom stack annotations, e.g. '1,3,4,5,6,7'. Annotation list must be same length as sequences. .TP \fB\-\-rotate\-numbers\fR YES/NO Draw X\-axis numbers with vertical orientation (default: False). .TP \fB\-\-number\-interval\fR NUMBER Distance between numbers on X\-axis (default: 5) .TP \fB\-S\fR \fB\-\-yaxis\fR NUMBER Height of yaxis in units. (Default: Maximum value with uninformative prior.) .TP \fB\-Y\fR \fB\-\-show\-yaxis\fR YES/NO Display entropy scale along y\-axis? (default: True) .TP \fB\-y\fR \fB\-\-ylabel\fR TEXT Y\-axis label (default depends on plot type and units) .TP \fB\-E\fR \fB\-\-show\-ends\fR YES/NO Label the ends of the sequence? (default: False) .TP \fB\-P\fR \fB\-\-fineprint\fR TEXT The fine print (default: weblogo version) .TP \fB\-\-ticmarks\fR NUMBER Distance between ticmarks (default: 1.0) .TP \fB\-\-errorbars\fR YES/NO Display error bars? (default: True) .TP \fB\-\-reverse\-stacks\fR YES/NO Draw stacks with largest letters on top? (default: True) .IP Color Options: .IP Colors can be specified using CSS2 syntax. e.g. 'red', '#FF0000', etc .TP \fB\-c\fR \fB\-\-color\-scheme\fR SCHEME Specify a standard color scheme (auto, base pairing, charge, chemistry, classic, hydrophobicity, monochrome) .TP \fB\-C\fR \fB\-\-color\fR COLOR SYMBOLS DESCRIPTION Specify symbol colors, e.g. \fB\-\-color\fR black AG \&'Purine' \fB\-\-color\fR red TC 'Pyrimidine' .TP \fB\-\-default\-color\fR COLOR Symbol color if not otherwise specified. .IP Font Format Options: .IP These options provide control over the font sizes and types. .TP \fB\-\-fontsize\fR POINTS Regular text font size in points (default: 10) .TP \fB\-\-title\-fontsize\fR POINTS Title text font size in points (default: 12) .TP \fB\-\-small\-fontsize\fR POINTS Small text font size in points (default: 6) .TP \fB\-\-number\-fontsize\fR POINTS Axis numbers font size in points (default: 8) .TP \fB\-\-text\-font\fR FONT Specify font for labels (default: ArialMT) .TP \fB\-\-logo\-font\fR FONT Specify font for logo (default: Arial\-BoldMT) .TP \fB\-\-title\-font\fR FONT Specify font for title (default: ArialMT) .IP Advanced Format Options: .IP These options provide fine control over the display of the logo. .TP \fB\-W\fR \fB\-\-stack\-width\fR POINTS Width of a logo stack (default: 10.8) .TP \fB\-\-aspect\-ratio\fR POINTS Ratio of stack height to width (default: 5) .TP \fB\-\-box\fR YES/NO Draw boxes around symbols? (default: no) .TP \fB\-\-resolution\fR DPI Bitmap resolution in dots per inch (DPI). (Default: 96 DPI, except png_print, 600 DPI) Low resolution bitmaps (DPI<300) are antialiased. .TP \fB\-\-scale\-width\fR YES/NO Scale the visible stack width by the fraction of symbols in the column? (I.e. columns with many gaps of unknowns are narrow.) (Default: yes) .TP \fB\-\-debug\fR YES/NO Output additional diagnostic information. (Default: False) .TP \fB\-\-errorbar\-fraction\fR NUMBER Sets error bars display proportion (default: 0.9) .TP \fB\-\-errorbar\-width\-fraction\fR NUMBER Sets error bars width display proportion (default: 0.25) .TP \fB\-\-errorbar\-gray\fR NUMBER Sets error bars' gray scale percentage (default: 0.75) .IP WebLogo Server: .IP Run a standalone webserver on a local port. .TP \fB\-\-serve\fR Start a standalone WebLogo server for creating sequence logos. .TP \fB\-\-port\fR PORT Listen to this local port. (Default: 8080)