.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.16. .TH AVERAGE_NUCLEOTIDE_IDENTITY.PY "1" "July 2021" "average_nucleotide_identity.py 0.2.11" "User Commands" .SH NAME average_nucleotide_identity.py \- average nucleotide identity .SH DESCRIPTION usage: average_nucleotide_identity.py [\-h] [\-\-version] \fB\-o\fR OUTDIRNAME \fB\-i\fR .TP INDIRNAME [\-v] [\-f] [\-s FRAGSIZE] [\-l LOGFILE] [\-\-skip_nucmer] [\-\-skip_blastn] [\-\-noclobber] [\-\-nocompress] [\-g] [\-\-gformat GFORMAT] [\-\-gmethod {mpl,seaborn}] [\-\-labels LABELS] [\-\-classes CLASSES] [\-m {ANIm,ANIb,ANIblastall,TETRA}] [\-\-scheduler {multiprocessing,SGE}] [\-\-workers WORKERS] [\-\-SGEgroupsize SGEGROUPSIZE] [\-\-SGEargs SGEARGS] [\-\-maxmatch] [\-\-nucmer_exe NUCMER_EXE] [\-\-filter_exe FILTER_EXE] [\-\-blastn_exe BLASTN_EXE] [\-\-makeblastdb_exe MAKEBLASTDB_EXE] [\-\-blastall_exe BLASTALL_EXE] [\-\-formatdb_exe FORMATDB_EXE] [\-\-write_excel] [\-\-rerender] [\-\-subsample SUBSAMPLE] [\-\-seed SEED] [\-\-jobprefix JOBPREFIX] .SS "optional arguments:" .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-\-version\fR show program's version number and exit .TP \fB\-o\fR OUTDIRNAME, \fB\-\-outdir\fR OUTDIRNAME Output directory (required) .TP \fB\-i\fR INDIRNAME, \fB\-\-indir\fR INDIRNAME Input directory name (required) .TP \fB\-v\fR, \fB\-\-verbose\fR Give verbose output .TP \fB\-f\fR, \fB\-\-force\fR Force file overwriting .TP \fB\-s\fR FRAGSIZE, \fB\-\-fragsize\fR FRAGSIZE Sequence fragment size for ANIb (default 1020) .TP \fB\-l\fR LOGFILE, \fB\-\-logfile\fR LOGFILE Logfile location .TP \fB\-\-skip_nucmer\fR Skip NUCmer runs, for testing (e.g. if output already present) .TP \fB\-\-skip_blastn\fR Skip BLASTN runs, for testing (e.g. if output already present) .TP \fB\-\-noclobber\fR Don't nuke existing files .TP \fB\-\-nocompress\fR Don't compress/delete the comparison output .TP \fB\-g\fR, \fB\-\-graphics\fR Generate heatmap of ANI .TP \fB\-\-gformat\fR GFORMAT Graphics output format(s) [pdf|png|jpg|svg] (default pdf,png,eps meaning three file formats) .TP \fB\-\-gmethod\fR {mpl,seaborn} Graphics output method (default mpl) .TP \fB\-\-labels\fR LABELS Path to file containing sequence labels .TP \fB\-\-classes\fR CLASSES Path to file containing sequence classes .TP \fB\-m\fR {ANIm,ANIb,ANIblastall,TETRA}, \fB\-\-method\fR {ANIm,ANIb,ANIblastall,TETRA} ANI method (default ANIm) .TP \fB\-\-scheduler\fR {multiprocessing,SGE} Job scheduler (default multiprocessing, i.e. locally) .TP \fB\-\-workers\fR WORKERS Number of worker processes for multiprocessing (default zero, meaning use all available cores) .TP \fB\-\-SGEgroupsize\fR SGEGROUPSIZE Number of jobs to place in an SGE array group (default 10000) .TP \fB\-\-SGEargs\fR SGEARGS Additional arguments for qsub .TP \fB\-\-maxmatch\fR Override MUMmer to allow all NUCmer matches .TP \fB\-\-nucmer_exe\fR NUCMER_EXE Path to NUCmer executable .TP \fB\-\-filter_exe\fR FILTER_EXE Path to delta\-filter executable .TP \fB\-\-blastn_exe\fR BLASTN_EXE Path to BLASTN+ executable .TP \fB\-\-makeblastdb_exe\fR MAKEBLASTDB_EXE Path to BLAST+ makeblastdb executable .TP \fB\-\-blastall_exe\fR BLASTALL_EXE Path to BLASTALL executable .TP \fB\-\-formatdb_exe\fR FORMATDB_EXE Path to BLAST formatdb executable .TP \fB\-\-write_excel\fR Write Excel format output tables .TP \fB\-\-rerender\fR Rerender graphics output without recalculation .TP \fB\-\-subsample\fR SUBSAMPLE Subsample a percentage [0\-1] or specific number (1\-n) of input sequences .TP \fB\-\-seed\fR SEED Set random seed for reproducible subsampling. .TP \fB\-\-jobprefix\fR JOBPREFIX Prefix for SGE jobs (default ANI). .SH AUTHOR This manpage was written by Nilesh Patra for the Debian distribution and can be used for any other usage of the program.