.\" DO NOT MODIFY THIS FILE!  It was generated by help2man 1.43.3.
.TH NTTHAL "1" "December 2013" "2.3.6" "Primer3 User Manuals"
.SH NAME
ntthal \- Provides Primer3's alignment functionality based on nearest-neighbor thermodynamical approach
.SH DESCRIPTION
\fBntthal\fR is analogous to \fBntdpal\fR.  Between two sequences, \fBntthal\fR finds
alignment/sec structure, that has the highest melting
temperature. Ntthal is based on nearest-neighbor thermodynamical
approach.
.SH SYNOPSIS
.B ntthal
\fIOPTIONS\fR oligo
.SH OPTIONS
\fB\-mv\fR monovalent_conc  \- concentration of monovalent cations in mM, by default 50 mM
.PP
\fB\-dv\fR divalent_conc    \- concentration of divalent cations in mM, by default 0 mM
.PP
\fB\-n\fR  dNTP_conc        \- concentration of deoxynycleotide triphosphate in mM, by default 0 mM
.PP
\fB\-d\fR  dna_conc         \- concentration of DNA strands in nM, by default 50 nM
.PP
\fB\-a\fR  mode             \- alignment type, END1, END2, ANY and HAIRPIN, by default ANY (when duplex)
.PP
\fB\-t\fR  temp             \- temperature at which duplex is calculated, by default 37C
.PP
\fB\-r\fR                   \- causes the alignment NOT to be displayed on stderr, _only_ Tm is printed
.PP
\fB\-maxloop\fR size        \- the maximum size of secondary structures loops.
.IP
Default is 30 (this is maximum allowed length, currently).
.PP
\fB\-path\fR <path>         \- the path to the thermodynamic parameter files
.PP
\fB\-s1\fR DNA_oligomer
.PP
\fB\-s2\fR DNA_oligomer
.SH AUTHORS
This manual page was created by Andreas Tille <tille@debian.org> using help2man
for Debian but can be freely used for any other purpose