.\" Automatically generated by Pod::Man 4.14 (Pod::Simple 3.40) .\" .\" Standard preamble: .\" ======================================================================== .de Sp \" Vertical space (when we can't use .PP) .if t .sp .5v .if n .sp .. .de Vb \" Begin verbatim text .ft CW .nf .ne \\$1 .. .de Ve \" End verbatim text .ft R .fi .. .\" Set up some character translations and predefined strings. \*(-- will .\" give an unbreakable dash, \*(PI will give pi, \*(L" will give a left .\" double quote, and \*(R" will give a right double quote. \*(C+ will .\" give a nicer C++. Capital omega is used to do unbreakable dashes and .\" therefore won't be available. \*(C` and \*(C' expand to `' in nroff, .\" nothing in troff, for use with C<>. .tr \(*W- .ds C+ C\v'-.1v'\h'-1p'\s-2+\h'-1p'+\s0\v'.1v'\h'-1p' .ie n \{\ . ds -- \(*W- . ds PI pi . if (\n(.H=4u)&(1m=24u) .ds -- \(*W\h'-12u'\(*W\h'-12u'-\" diablo 10 pitch . if (\n(.H=4u)&(1m=20u) .ds -- \(*W\h'-12u'\(*W\h'-8u'-\" diablo 12 pitch . ds L" "" . ds R" "" . ds C` "" . ds C' "" 'br\} .el\{\ . ds -- \|\(em\| . ds PI \(*p . ds L" `` . ds R" '' . ds C` . ds C' 'br\} .\" .\" Escape single quotes in literal strings from groff's Unicode transform. .ie \n(.g .ds Aq \(aq .el .ds Aq ' .\" .\" If the F register is >0, we'll generate index entries on stderr for .\" titles (.TH), headers (.SH), subsections (.SS), items (.Ip), and index .\" entries marked with X<> in POD. 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No user-serviceable parts. . \" fudge factors for nroff and troff .if n \{\ . ds #H 0 . ds #V .8m . ds #F .3m . ds #[ \f1 . ds #] \fP .\} .if t \{\ . ds #H ((1u-(\\\\n(.fu%2u))*.13m) . ds #V .6m . ds #F 0 . ds #[ \& . ds #] \& .\} . \" simple accents for nroff and troff .if n \{\ . ds ' \& . ds ` \& . ds ^ \& . ds , \& . ds ~ ~ . ds / .\} .if t \{\ . ds ' \\k:\h'-(\\n(.wu*8/10-\*(#H)'\'\h"|\\n:u" . ds ` \\k:\h'-(\\n(.wu*8/10-\*(#H)'\`\h'|\\n:u' . ds ^ \\k:\h'-(\\n(.wu*10/11-\*(#H)'^\h'|\\n:u' . ds , \\k:\h'-(\\n(.wu*8/10)',\h'|\\n:u' . ds ~ \\k:\h'-(\\n(.wu-\*(#H-.1m)'~\h'|\\n:u' . ds / \\k:\h'-(\\n(.wu*8/10-\*(#H)'\z\(sl\h'|\\n:u' .\} . \" troff and (daisy-wheel) nroff accents .ds : \\k:\h'-(\\n(.wu*8/10-\*(#H+.1m+\*(#F)'\v'-\*(#V'\z.\h'.2m+\*(#F'.\h'|\\n:u'\v'\*(#V' .ds 8 \h'\*(#H'\(*b\h'-\*(#H' .ds o \\k:\h'-(\\n(.wu+\w'\(de'u-\*(#H)/2u'\v'-.3n'\*(#[\z\(de\v'.3n'\h'|\\n:u'\*(#] .ds d- \h'\*(#H'\(pd\h'-\w'~'u'\v'-.25m'\f2\(hy\fP\v'.25m'\h'-\*(#H' .ds D- D\\k:\h'-\w'D'u'\v'-.11m'\z\(hy\v'.11m'\h'|\\n:u' .ds th \*(#[\v'.3m'\s+1I\s-1\v'-.3m'\h'-(\w'I'u*2/3)'\s-1o\s+1\*(#] .ds Th \*(#[\s+2I\s-2\h'-\w'I'u*3/5'\v'-.3m'o\v'.3m'\*(#] .ds ae a\h'-(\w'a'u*4/10)'e .ds Ae A\h'-(\w'A'u*4/10)'E . \" corrections for vroff .if v .ds ~ \\k:\h'-(\\n(.wu*9/10-\*(#H)'\s-2\u~\d\s+2\h'|\\n:u' .if v .ds ^ \\k:\h'-(\\n(.wu*10/11-\*(#H)'\v'-.4m'^\v'.4m'\h'|\\n:u' . \" for low resolution devices (crt and lpr) .if \n(.H>23 .if \n(.V>19 \ \{\ . ds : e . ds 8 ss . ds o a . ds d- d\h'-1'\(ga . ds D- D\h'-1'\(hy . ds th \o'bp' . ds Th \o'LP' . ds ae ae . ds Ae AE .\} .rm #[ #] #H #V #F C .\" ======================================================================== .\" .IX Title "GETCDS 1" .TH GETCDS 1 "2021-08-15" "perl v5.32.1" "User Contributed Perl Documentation" .\" For nroff, turn off justification. Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" getcds.pl \- CDS extraction from various annotation formats .SH "SYNOPSIS" .IX Header "SYNOPSIS" getcds.pl [options] input file [output file] [input file [outputfile] ...] .SH "OPTIONS" .IX Header "OPTIONS" .Vb 3 \& Options include: \& redirect => Enable a redirect at runtime. explained below. \& force => allows writing empty .cds files .Ve .PP If [output file] is unspecificied, \*(L"[input file].cds\*(R" is used. .PP There's lots of dark magic that can be performed with .redirect files. Redirects are used to modify the ordinary behaviour and can be applied on a global basis (a .redirect file in the same directory as [input file]), and/or on a per-input basis ([input file].redirect). Redirects are read in that order such that per-input effects are applied ontop of global effects. .PP If you've left the filename as-is from an Ensembl downoad, the appropriate Ensembl \s-1DB\s0 source will be guessed automatically. .PP Possible redirects: .IP "\s-1CDS\s0 \- changes what tag type is searched for when running on bioperl files (default is \s-1CDS\s0)" 4 .IX Item "CDS - changes what tag type is searched for when running on bioperl files (default is CDS)" .PD 0 .IP "offset \- add some value to all coordinates" 4 .IX Item "offset - add some value to all coordinates" .IP "+bioperl \- on top of any other redirect-based data, also extract annotation via bioperl (using a direct disables bioperl's parser by default)" 4 .IX Item "+bioperl - on top of any other redirect-based data, also extract annotation via bioperl (using a direct disables bioperl's parser by default)" .IP "ensembl_build \- grab data from the ensembl (in the specified schema)" 4 .IX Item "ensembl_build - grab data from the ensembl (in the specified schema)" .IP "ucsc_build \- grab data from (in the specified schema)" 4 .IX Item "ucsc_build - grab data from (in the specified schema)" .IP "murasaki_synth \- synthesize annotation data for each anchor from an alignment (if this is a directory, then each input file (eg input.lav) is checked for a corresponding .anchors file (eg. input.anchors)." 4 .IX Item "murasaki_synth - synthesize annotation data for each anchor from an alignment (if this is a directory, then each input file (eg input.lav) is checked for a corresponding .anchors file (eg. input.anchors)." .IP "primary \- what to type of tags to create from data gathered from outside data (eg: ensembl, ucsc, or murasaki). default is the same as the cds redirect." 4 .IX Item "primary - what to type of tags to create from data gathered from outside data (eg: ensembl, ucsc, or murasaki). default is the same as the cds redirect." .IP "chromosome \- forces a specific chromosome rather than deriving from filename" 4 .IX Item "chromosome - forces a specific chromosome rather than deriving from filename" .IP "gtf \- extract annotation from a .gtf file" 4 .IX Item "gtf - extract annotation from a .gtf file"