|GENOME-MUSIC-CLINICAL-CORRELATION(1p)||User Contributed Perl Documentation||GENOME-MUSIC-CLINICAL-CORRELATION(1p)|
genome music clinical-correlation¶
genome music clinical-correlation - Correlate phenotypic traits against mutated genes, or against individual variants
This document describes genome music clinical-correlation version 0.04 (2018-07-05 at 09:17:13)
genome music clinical-correlation --bam-list=? --output-file=? [--maf-file=?] [--glm-clinical-data-file=?] [--use-maf-in-glm] [--skip-non-coding] [--skip-silent] [--clinical-correlation-matrix-file=?] [--input-clinical-correlation-matrix-file=?] [--genetic-data-type=?] [--numeric-clinical-data-file=?] [--numerical-data-test-method=?] [--categorical-clinical-data-file=?] [--glm-model-file=?]
... music clinical-correlation \ --bam-list /path/myBamList.tsv \ --maf-file /path/myMAF.tsv \ --numeric-clinical-data-file /path/myNumericData.tsv \ --genetic-data-type 'gene' \ --output-file /path/output_file ... music clinical-correlation \ --maf-file /path/myMAF.tsv \ --bam-list /path/myBamList.tsv \ --numeric-clinical-data-file /path/myNumericData.tsv \ --categorical-clinical-data-file /path/myClassData.tsv \ --genetic-data-type 'gene' \ --output-file /path/output_file ... music clinical-correlation \ --maf-file /path/myMAF.tsv \ --bam-list /path/myBamList.tsv \ --output-file /path/output_file \ --glm-model-file /path/model.tsv \ --glm-clinical-data-file /path/glm_clinical_data.tsv \ --use-maf-in-glm
- maf-file Text
- List of mutations using TCGA MAF specification v2.3
- glm-clinical-data-file Text
- Clinical traits, mutational profiles, other mixed clinical data (See DESCRIPTION)
- use-maf-in-glm Boolean
- Create a variant matrix from the MAF file as variant input to GLM
Default value 'false' (--nouse-maf-in-glm) if not specified
- nouse-maf-in-glm Boolean
- Make use-maf-in-glm 'false'
- skip-non-coding Boolean
- Skip non-coding mutations from the provided MAF file
Default value 'true' if not specified
- noskip-non-coding Boolean
- Make skip-non-coding 'false'
- skip-silent Boolean
- Skip silent mutations from the provided MAF file
Default value 'true' if not specified
- noskip-silent Boolean
- Make skip-silent 'false'
- clinical-correlation-matrix-file Text
- Specify a file to store the sample-vs-gene matrix created during calculations
- input-clinical-correlation-matrix-file Text
- Instead of creating this from the MAF, input the sample-vs-gene matrix for calculations
- genetic-data-type Text
- Correlate clinical data to "gene" or "variant" level
Default value 'gene' if not specified
- numeric-clinical-data-file Text
- Table of samples (y) vs. numeric clinical data category (x)
- numerical-data-test-method Text
- Either 'cor' for Pearson Correlation or 'wilcox' for the Wilcoxon Rank-Sum
Test for numerical clinical data
Default value 'cor' if not specified
- categorical-clinical-data-file Text
- Table of samples (y) vs. categorical clinical data category (x)
- glm-model-file Text
- File outlining the type of model, response variable, covariants, etc. for the GLM analysis. (See DESCRIPTION)
This command relates clinical traits and mutational data. Either one can perform correlation analysis between mutations recorded in a MAF and the particular phenotypic traits recorded in clinical data files for the same samples, or one can run a generalized linear model (GLM) analysis on the same types of data.
The clinical data files for correlation must be separated between numeric and categoric data and must follow these conventions:
- Headers are required
- Each file must include at least 1 sample_id column and 1 attribute column, with the format being [sample_id clinical_data_attribute_1 clinical_data_attribute_2 ...]
- The sample ID must match the sample ID listed in the MAF under "Tumor_Sample_Barcode" for relating the mutations of this sample.
Note the importance of the headers: the header for each clinical_data_attribute will appear in the output file to denote relationships with the mutation data from the MAF.
Internally, the input data is fed into an R script which calculates a P-value representing the probability that the correlation seen between the mutations in each gene (or variant) and each phenotype trait are random. Lower P-values indicate lower randomness, or likely true correlations.
The results are saved to the output filename given with a suffix appended; ".numeric.csv" will be appended for results derived from numeric clinical data, and ".categorical.csv" will be appended for results derived from categorical clinical data. Also, ".glm.csv" will be appended to the output filename for GLM results.
The GLM analysis accepts a mixed numeric and categoric clinical data file, input using the parameter --glm-clinical-data-file. GLM clinical data must adhere to the formats described above for the correlation clinical data files. GLM also requires the user to input a --glm-model-file. This file requires specific headers and defines the analysis to be performed rather exactly. Here are the conventions required for this file:
- Columns must be ordered as such:
- [ analysis_type clinical_data_trait_name variant/gene_name covariates memo ]
- The 'analysis_type' column must contain either "Q", indicating a quantative trait, or "B", indicating a binary trait will be examined.
- The 'clinical_data_trait_name' is the name of a clinical data trait defined by being a header in the --glm-clinical-data-file.
- The 'variant/gene_name' can either be the name of one or more columns from the --glm-clinical-data-file, or the name of one or more mutated gene names from the MAF, separated by "|". If this column is left blank, or instead contains "NA", then each column from either the variant mutation matrix (--use-maf-in-glm) or alternatively the --glm-clinical-data-file is used consecutively as the variant column in independent analyses.
- 'covariates' are the names of one or more columns from the --glm-clinical-data-file, separated by "+".
- 'memo' is any note deemed useful to the user. It will be printed in the output data file for reference.
GLM analysis may be performed using solely the data input into --glm-clinical-data-file, as described above, or alternatively, mutational data from the MAF may be included as variants in the GLM analysis, as also described above. Use the --use-maf-in-glm flag to include the mutation matrix derived from the maf as variant data.
Note that all input files for both correlation and GLM analysis must be tab-separated.
- Provide a file containing sample names and normal/tumor BAM locations for each. Use the tab- delimited format [sample_name normal_bam tumor_bam] per line. This tool only needs sample_name, so all other columns can be skipped. The sample_name must be the same as the tumor sample names used in the MAF file (16th column, with the header Tumor_Sample_Barcode).
Copyright (C) 2010-2011 Washington University in St. Louis.
It is released under the Lesser GNU Public License (LGPL) version 3. See the associated LICENSE file in this distribution.
Nathan D. Dees, Ph.D. Qunyuan Zhang, Ph.D. William Schierding, M.S.