.\" Automatically generated by Pod::Man 4.09 (Pod::Simple 3.35) .\" .\" Standard preamble: .\" ======================================================================== .de Sp \" Vertical space (when we can't use .PP) .if t .sp .5v .if n .sp .. .de Vb \" Begin verbatim text .ft CW .nf .ne \\$1 .. .de Ve \" End verbatim text .ft R .fi .. .\" Set up some character translations and predefined strings. \*(-- will .\" give an unbreakable dash, \*(PI will give pi, \*(L" will give a left .\" double quote, and \*(R" will give a right double quote. \*(C+ will .\" give a nicer C++. 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Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" bp_process_wormbase.pl \- Massage WormBase GFF files into a version suitable for the Generic Genome Browser .SH "SYNOPSIS" .IX Header "SYNOPSIS" .Vb 1 \& % bp_process_wormbase.pl ./WS61 > wormbase.gff .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" This script massages the Wormbase \s-1GFF\s0 files located at ftp://www.wormbase.org/pub/wormbase/GENE_DUMPS into a version of the \&\s-1GFF\s0 format suitable for display by the generic genome browser. It mainly adds comments to the annotations and designates certain well-spaced genetic loci as framework landmarks. .PP This script requires the AcePerl distribution, which is available on \&\s-1CPAN\s0 (look for the \*(L"Ace\*(R" module). .PP To use this script, get the WormBase \s-1GFF\s0 files from the \s-1FTP\s0 site listed above and place them in a directory. It might be a good idea to name the directory after the current release, such as \s-1WS61.\s0 You do not need to uncompress the files. .PP Then give that directory as the argument to this script and capture the script's output to a file: .PP .Vb 1 \& % bp_process_wormbase.pl ./WS61 > wormbase.gff .Ve .PP It may take a while before you see output from this script, since it must first fetch gene and protein database from the remote AceDB running at www.wormbase.org. The wormbase.gff file can then be loaded into a Bio::DB::GFF database using the following command: .PP .Vb 1 \& % bulk_load_gff.pl \-d wormbase.gff .Ve .SH "SEE ALSO" .IX Header "SEE ALSO" Bio::DB::GFF, bulk_load_gff.pl, load_gff.pl .SH "AUTHOR" .IX Header "AUTHOR" Lincoln Stein .PP Copyright (c) 2002 Cold Spring Harbor Laboratory .PP This library is free software; you can redistribute it and/or modify it under the same terms as Perl itself. See \s-1DISCLAIMER\s0.txt for disclaimers of warranty.