.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.46.4. .TH LOCUSPOCUS "1" "January 2016" "locuspocus 0.15.1" "User Commands" .SH NAME locuspocus \- calculate locus coordinates for the given gene annotation .SH SYNOPSIS .B locuspocus [\fI\,options\/\fR] \fI\,gff3file1 \/\fR[\fI\,gff3file2 gff3file3 \/\fR...] .SH DESCRIPTION Basic options: .TP \fB\-d\fR|\-\-debug print detailed debugging messages to terminal (standard error) .TP \fB\-h\fR|\-\-help print this help message and exit .TP \fB\-v\fR|\-\-version print version number and exit .IP iLocus parsing: .TP \fB\-l\fR|\-\-delta: INT when parsing interval loci, use the following delta to extend gene loci and include potential regulatory regions; default is 500 .TP \fB\-s\fR|\-\-skipends when enumerating interval loci, exclude unannotated (and presumably incomplete) iLoci at either end of the sequence .TP \fB\-e\fR|\-\-endsonly report only incomplete iLocus fragments at the unannotated ends of sequences (complement of \fB\-\-skipends\fR) .TP \fB\-y\fR|\-\-skipiiloci do not report intergenic iLoci .IP Refinement options: .TP \fB\-r\fR|\-\-refine by default genes are grouped in the same iLocus if they have any overlap; 'refine' mode allows for a more nuanced handling of overlapping genes .TP \fB\-c\fR|\-\-cds use CDS rather than UTRs for determining gene overlap; implies 'refine' mode .TP \fB\-m\fR|\-\-minoverlap: INT the minimum number of nucleotides two genes must overlap to be grouped in the same iLocus; default is 1 .IP Output options: .TP \fB\-n\fR|\-\-namefmt: STR provide a printf\-style format string to override the default ID format for newly created loci; default is 'locus%lu' (locus1, locus2, etc) for loci and 'iLocus%lu' (iLocus1, iLocus2, etc) for interval loci; note the format string should include a single %lu specifier to be filled in with a long unsigned integer value .TP \fB\-i\fR|\-\-ilens: FILE create a file with the lengths of each intergenic iLocus .TP \fB\-g\fR|\-\-genemap: FILE print a mapping from each gene annotation to its corresponding locus to the given file .TP \fB\-o\fR|\-\-outfile: FILE name of file to which results will be written; default is terminal (standard output) .TP \fB\-T\fR|\-\-retainids retain original feature IDs from input files; conflicts will arise if input contains duplicated ID values .TP \fB\-t\fR|\-\-transmap: FILE print a mapping from each transcript annotation to its corresponding locus to the given file .TP \fB\-V\fR|\-\-verbose include all locus subfeatures (genes, RNAs, etc) in the GFF3 output; default includes only locus features .IP Input options: .TP \fB\-f\fR|\-\-filter: TYPE comma\-separated list of feature types to use in constructing loci/iLoci; default is 'gene' .TP \fB\-p\fR|\-\-parent: CT:PT if a feature of type $CT exists without a parent, create a parent for this feature with type $PT; for example, mRNA:gene will create a gene feature as a parent for any top\-level mRNA feature; this option can be specified multiple times .TP \fB\-u\fR|\-\-pseudo correct erroneously labeled pseudogenes