.TH "TQS" "1" "January 2008" .SH "NAME" tqs \- Trim Quality Solexa-Illumina Sequences .SH SYNOPSIS Quality trim solexa-Illumina sequence reads using user-defined thresholds. .SH "OPTIONS" .PP .IP "\-h, \-\-help " 10 show this help message and exit .IP "\-f SEQFILE, \-\-sequence file=SEQFILE" 10 Illumina sequence file - Output format from the 1G Genome Analyzer (_seq.txt): 7 1 255 669 AACCCCCACTCCTACAACGCCATCATTCCCCTCGAC .IP "\-q QUALFILE, \-\-qual file=QUALFILE " 10 A prb file containing all the Illumina intensities, as outputted by the 1G Genome Analyzer (_prb.txt) .IP "\-l MER, \-\-length=MER " 10 Length of sequence reads (i.e. Number of sequencing cycles, default=36) .IP "\-t THRESHOLD, \-\-threshold=THRESHOLD" 10 Base intensity threshold value (\-40 to 40, default=5) .IP "\-d DIFF, \-\-difference=DIFF " 10 Base intensity difference between top intensity and second best (1 to 80, default=5) .IP "\-c CONSEC, \-\-consec=CONSEC " 10 Minimum number of consecutive bases passing threshold values (default=20) .IP "\-v, \-\-verbose " 10 Runs in Verbose mode. .SH SEE ALSO /usr/share/doc/ssake/TQS.readme .SH "AUTHORS" .PP This manual page was written by Andreas Tille for the \fBDebian\fP system (but may be used by others). Permission is granted to copy, distribute and/or modify this document under the terms of the GNU General Public License, Version 2 any later version published by the Free Software Foundation. .PP On Debian systems, the complete text of the GNU General Public License can be found in /usr/share/common-licenses/GPL.