.\" .\" $Id: 6ft.1,v 1.2 2003/08/11 12:09:14 vflegel Exp $ .\" Copyright (c) 2003 SIB Swiss Institute of Bioinformatics .\" Process this file with .\" groff -man -Tascii .\" for ascii output or .\" groff -man -Tps .\" for postscript output .\" .TH 6FT 1 "May 2003" "pftools 2.3" "pftools" .\" ------------------------------------------------ .\" Name section .\" ------------------------------------------------ .SH NAME 6ft \- 6-frame translation of a DNA sequence into protein .\" ------------------------------------------------ .\" Synopsis section .\" ------------------------------------------------ .SH SYNOPSIS .TP 10 .B 6ft [ .B \-h ] [ .B \-r | .B \-s ] [ .B \-W .I width ] .B < .I sequence_library .\" ------------------------------------------------ .\" Description section .\" ------------------------------------------------ .SH DESCRIPTION .B 6ft translates all six reading frames of a double-stranded DNA sequence into individual protein sequences. Stop codons are represented by character .RB \(dq O \(dq. The program .B 6ft reads input fasta formatted DNA sequences from standard input. If the sequences are contained in a file, the input can be redirected from this file using the shell's input redirector .RB ' < ' as shown in the .I synopsis section. The .I sequence_library contains one or several sequences in Pearson/Fasta format. .LP This program may be used for searching genomic or EST sequence databases with a protein profile. Better results are usually obtained by first converting the protein profile into a frame-search profile, and then searching a 2-frame translation of the DNA sequence database generated with pftools program .BR 2ft (1), .I see manual pages of pftools programs .BR ptof (1) and .BR 2ft (1). .\" ------------------------------------------------ .\" Options section .\" ------------------------------------------------ .SH OPTIONS .\" --- h --- .TP .B \-h Display usage help text. .\" --- r --- .TP .B \-r Translate only reverse (antisense) strand. .br This option is mutualy exclusive with option .BR \-s . .br Default: translate both strands .\" --- s --- .TP .B \-s Translate only sense strand. .br This option is mutualy exclusive with option .BR \-r . .br Default: translate both strands .\" --- W --- .TP .BI \-W\ width Set sequence output width. .br The value of .RI ' width ' specifies how many residues will be output on one line. .br Type: integer .br Default: 60 .\" ------------------------------------------------ .\" Examples section .\" ------------------------------------------------ .SH EXAMPLES .I See manual pages of pftools program .BR ptof (1). .\" ------------------------------------------------ .\" See also section .\" ------------------------------------------------ .SH "SEE ALSO" .BR ptof (1), .BR pfsearch (1), .BR pfscan (1) .\" ------------------------------------------------ .\" Author section .\" ------------------------------------------------ .SH AUTHOR The .B pftools package was developed by Philipp Bucher. .br Any comments or suggestions should be addressed to .