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.IX Title "Bio::Tools::Run::Phylo::Njtree::Best 3pm"
.TH Bio::Tools::Run::Phylo::Njtree::Best 3pm "2023-01-22" "perl v5.36.0" "User Contributed Perl Documentation"
.\" For nroff, turn off justification.  Always turn off hyphenation; it makes
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.nh
.SH "NAME"
Bio::Tools::Run::Phylo::Njtree::Best \- Wrapper around the Njtree (Njtree/phyml) best program.
.SH "SYNOPSIS"
.IX Header "SYNOPSIS"
.Vb 3
\&  use Bio::Tools::Run::Phylo::Njtree::Best;
\&  use Bio::AlignIO;
\&  use Bio::TreeIO;
\&
\&  my $alignio = Bio::AlignIO\->new(\-format => \*(Aqfasta\*(Aq,
\&                                 \-file   => \*(Aqt/data/njtree_aln2.nucl.mfa\*(Aq);
\&
\&  my $aln = $alignio\->next_aln;
\&
\&  my $treeio = Bio::TreeIO\->new(
\&      \-format => \*(Aqnhx\*(Aq, \-file => \*(Aqt/data/species_tree_njtree.nh\*(Aq);
\&
\&  my $tree = $treeio\->next_tree;
\&
\&  my $njtree_best = Bio::Tools::Run::Phylo::Njtree::Best\->new();
\&  $njtree_best\->alignment($aln);
\&  $njtree_best\->tree($tree);
\&  my $nhx_tree = $njtree_best\->run();
.Ve
.SH "DESCRIPTION"
.IX Header "DESCRIPTION"
This is a wrapper around the best program of Njtree by Li Heng.  See
http://treesoft.sourceforge.net/njtree.shtml for more information.
.PP
Wrapper for the calculation of a reconciled phylogenetic tree with
inferred duplication tags from amultiple sequence alignment and a
species tree using \s-1NJTREE.\s0
.SS "Helping the module find your executable"
.IX Subsection "Helping the module find your executable"
You will need to enable \s-1NJTREEDIR\s0 to find the njtree program. This can be
done in (at least) three ways:
.PP
.Vb 8
\&  1. Make sure the njtree executable is in your path (i.e. 
\&     \*(Aqwhich njtree\*(Aq returns a valid program
\&  2. define an environmental variable NJTREEDIR which points to a 
\&     directory containing the \*(Aqnjtree\*(Aq app:
\&   In bash 
\&        export NJTREEDIR=/home/progs/treesoft/njtree   or
\&   In csh/tcsh
\&        setenv NJTREEDIR /home/progs/treesoft/njtree
\&
\&  3. include a definition of an environmental variable NJTREEDIR 
\&      in every script that will
\&     BEGIN {$ENV{NJTREEDIR} = \*(Aq/home/progs/treesoft/njtree\*(Aq; }
\&     use Bio::Tools::Run::Phylo::Njtree::Best;
.Ve
.SH "FEEDBACK"
.IX Header "FEEDBACK"
.SS "Mailing Lists"
.IX Subsection "Mailing Lists"
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists.  Your participation is much appreciated.
.PP
.Vb 2
\&  bioperl\-l@bioperl.org                  \- General discussion
\&  http://bioperl.org/wiki/Mailing_lists  \- About the mailing lists
.Ve
.SS "Support"
.IX Subsection "Support"
Please direct usage questions or support issues to the mailing list:
.PP
\&\fIbioperl\-l@bioperl.org\fR
.PP
rather than to the module maintainer directly. Many experienced and 
reponsive experts will be able look at the problem and quickly 
address it. Please include a thorough description of the problem 
with code and data examples if at all possible.
.SS "Reporting Bugs"
.IX Subsection "Reporting Bugs"
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.  Bug reports can be submitted via the web:
.PP
.Vb 1
\& http://redmine.open\-bio.org/projects/bioperl/
.Ve
.SH "AUTHOR \-  Albert Vilella"
.IX Header "AUTHOR - Albert Vilella"
Email avilella-at-gmail-dot-com
.SH "APPENDIX"
.IX Header "APPENDIX"
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
.SS "program_name"
.IX Subsection "program_name"
.Vb 5
\& Title   : program_name
\& Usage   : $factory\->program_name()
\& Function: holds the program name
\& Returns:  string
\& Args    : None
.Ve
.SS "program_dir"
.IX Subsection "program_dir"
.Vb 5
\& Title   : program_dir
\& Usage   : $factory\->program_dir(@params)
\& Function: returns the program directory, obtained from ENV variable.
\& Returns:  string
\& Args    :
.Ve
.SS "new"
.IX Subsection "new"
.Vb 9
\& Title   : new
\& Usage   : my $njtree_best = Bio::Tools::Run::Phylo::Njtree::Best\->new();
\& Function: Builds a new Bio::Tools::Run::Phylo::Njtree::Best
\& Returns : Bio::Tools::Run::Phylo::Njtree::Best
\& Args    : \-alignment => the Bio::Align::AlignI object
\&           \-tree => the Bio::Tree::TreeI object
\&           \-save_tempfiles => boolean to save the generated tempfiles and
\&                              NOT cleanup after onesself (default FALSE)
\&           \-executable => where the njtree executable resides
.Ve
.PP
See also: Bio::Tree::TreeI, Bio::Align::AlignI
.SS "prepare"
.IX Subsection "prepare"
.Vb 7
\& Title   : prepare
\& Usage   : my $rundir = $njtree_best\->prepare();
\& Function: prepare the njtree_best analysis using the default or updated parameters
\&           the alignment parameter and species tree must have been set
\& Returns : value of rundir
\& Args    : L<Bio::Align::AlignI> object,
\&           L<Bio::Tree::TreeI> object [optional]
.Ve
.SS "run"
.IX Subsection "run"
.Vb 7
\& Title   : run
\& Usage   : my $nhx_tree = $njtree_best\->run();
\& Function: run the njtree_best analysis using the default or updated parameters
\&           the alignment parameter must have been set
\& Returns : L<Bio::Tree::TreeI> object [optional]
\& Args    : L<Bio::Align::AlignI> object
\&           L<Bio::Tree::TreeI> object
.Ve
.SS "error_string"
.IX Subsection "error_string"
.Vb 5
\& Title   : error_string
\& Usage   : $obj\->error_string($newval)
\& Function: Where the output from the last analysus run is stored.
\& Returns : value of error_string
\& Args    : newvalue (optional)
.Ve
.SS "version"
.IX Subsection "version"
.Vb 6
\& Title   : version
\& Usage   : exit if $prog\->version() < 1.8
\& Function: Determine the version number of the program
\& Example :
\& Returns : float or undef
\& Args    : none
.Ve
.SS "alignment"
.IX Subsection "alignment"
.Vb 8
\& Title   : alignment
\& Usage   : $njtree_best\->align($aln);
\& Function: Get/Set the L<Bio::Align::AlignI> object
\& Returns : L<Bio::Align::AlignI> object
\& Args    : [optional] L<Bio::Align::AlignI>
\& Comment : We could potentially add support for running directly on a file
\&           but we shall keep it simple
\& See also: L<Bio::SimpleAlign>
.Ve
.SS "tree"
.IX Subsection "tree"
.Vb 6
\& Title   : tree
\& Usage   : $njtree_best\->tree($tree, %params);
\& Function: Get/Set the L<Bio::Tree::TreeI> object
\& Returns : L<Bio::Tree::TreeI> 
\& Args    : [optional] $tree => L<Bio::Tree::TreeI>,
\&           [optional] %parameters => hash of tree\-specific parameters
\&
\& Comment : We could potentially add support for running directly on a file
\&           but we shall keep it simple
\& See also: L<Bio::Tree::Tree>
.Ve
.SS "check_names"
.IX Subsection "check_names"
.Vb 6
\& Title   : check_names
\& Usage   :
\& Function:
\& Example :
\& Returns : 
\& Args    :
.Ve
.SS "_setparams"
.IX Subsection "_setparams"
.Vb 7
\& Title   :  _setparams
\& Usage   :  Internal function, not to be called directly
\& Function:  Create parameter inputs for njtree_best program
\& Example :
\& Returns : parameter string to be passed to njtree_best
\&           during align or profile_align
\& Args    : name of calling object
.Ve
.SH "Bio::Tools::Run::BaseWrapper methods"
.IX Header "Bio::Tools::Run::BaseWrapper methods"
.SS "save_tempfiles"
.IX Subsection "save_tempfiles"
.Vb 5
\& Title   : save_tempfiles
\& Usage   : $obj\->save_tempfiles($newval)
\& Function: 
\& Returns : value of save_tempfiles
\& Args    : newvalue (optional)
.Ve
.SS "outfile_name"
.IX Subsection "outfile_name"
.Vb 6
\& Title   : outfile_name
\& Usage   : my $outfile = $njtree_best\->outfile_name();
\& Function: Get/Set the name of the output file for this run
\&           (if you wanted to do something special)
\& Returns : string
\& Args    : [optional] string to set value to
.Ve
.SS "tempdir"
.IX Subsection "tempdir"
.Vb 5
\& Title   : tempdir
\& Usage   : my $tmpdir = $self\->tempdir();
\& Function: Retrieve a temporary directory name (which is created)
\& Returns : string which is the name of the temporary directory
\& Args    : none
.Ve
.SS "cleanup"
.IX Subsection "cleanup"
.Vb 5
\& Title   : cleanup
\& Usage   : $njtree_best\->cleanup();
\& Function: Will cleanup the tempdir directory
\& Returns : none
\& Args    : none
.Ve
.SS "io"
.IX Subsection "io"
.Vb 5
\& Title   : io
\& Usage   : $obj\->io($newval)
\& Function:  Gets a L<Bio::Root::IO> object
\& Returns : L<Bio::Root::IO>
\& Args    : none
.Ve