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Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" Bio::Tools::Run::EMBOSSApplication \- class for EMBOSS Applications .SH "SYNOPSIS" .IX Header "SYNOPSIS" .Vb 5 \& # Get an EMBOSS factory \& use Bio::Factory::EMBOSS; \& $f = Bio::Factory::EMBOSS \-> new(); \& # Get an EMBOSS application object from the factory \& $water = $f\->program(\*(Aqwater\*(Aq) || die "Program not found!\en"; \& \& # Here is an example of running the application \- water can \& # compare 1 sequence against 1 or more sequences using Smith\-Waterman. \& # Pass a Sequence object and a reference to an array of objects. \& \& my $wateroutfile = \*(Aqout.water\*(Aq; \& $water\->run({\-asequence => $seq_object, \& \-bsequence => \e@seq_objects, \& \-gapopen => \*(Aq10.0\*(Aq, \& \-gapextend => \*(Aq0.5\*(Aq, \& \-outfile => $wateroutfile}); \& \& # Now you might want to get the alignment \& use Bio::AlignIO; \& my $alnin = Bio::AlignIO\->new(\-format => \*(Aqemboss\*(Aq, \& \-file => $wateroutfile); \& \& while ( my $aln = $alnin\->next_aln ) { \& # process the alignment \-\- these will be Bio::SimpleAlign objects \& } .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" The EMBOSSApplication class can represent any \s-1EMBOSS\s0 program. It is created by a Bio::Factory::EMBOSS object. .PP If you want to check command line options before sending them to the program set \f(CW$prog\fR\->verbose to positive integer. The \s-1ADC\s0 description of the available command line options is then parsed in and compared to input. .PP See also Bio::Factory::EMBOSS and Bio::Tools::Run::EMBOSSacd. .SH "FEEDBACK" .IX Header "FEEDBACK" .SS "Mailing Lists" .IX Subsection "Mailing Lists" User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated. .PP .Vb 2 \& bioperl\-l@bioperl.org \- General discussion \& http://bioperl.org/wiki/Mailing_lists \- About the mailing lists .Ve .SS "Support" .IX Subsection "Support" Please direct usage questions or support issues to the mailing list: .PP \&\fIbioperl\-l@bioperl.org\fR .PP rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. .SS "Reporting Bugs" .IX Subsection "Reporting Bugs" Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: .PP .Vb 1 \& http://redmine.open\-bio.org/projects/bioperl/ .Ve .SH "AUTHOR \- Heikki Lehvaslaiho" .IX Header "AUTHOR - Heikki Lehvaslaiho" Email heikki-at-bioperl-dot-org .SS "\s-1CONTRIBUTORS\s0" .IX Subsection "CONTRIBUTORS" Email: jason\-AT\-bioperl_DOT_org .SH "APPENDIX" .IX Header "APPENDIX" The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ .SS "run" .IX Subsection "run" .Vb 5 \& Title : run \& Usage : $embossapplication\->run($attribute_hash) \& Function: Runs the EMBOSS program. \& Returns : string or creates files for now; will return objects! \& Args : hash of input to the program .Ve .SS "acd" .IX Subsection "acd" .Vb 8 \& Title : acd \& Usage : $embossprogram\->acd \& Function: finds out all the possible qualifiers for this \& EMBOSS application. They can be used to debug the \& options given. \& Throws : \& Returns : boolean \& Args : .Ve .SS "name" .IX Subsection "name" .Vb 8 \& Title : name \& Usage : $embossprogram\->name \& Function: sets/gets the name of the EMBOSS program \& Setting is done by the EMBOSSFactory object, \& you should only get it. \& Throws : \& Returns : name string \& Args : None .Ve .SS "descr" .IX Subsection "descr" .Vb 8 \& Title : descr \& Usage : $embossprogram\->descr \& Function: sets/gets the descr of the EMBOSS program \& Setting is done by the EMBOSSFactory object, \& you should only get it. \& Throws : \& Returns : description string \& Args : None .Ve .SS "group" .IX Subsection "group" .Vb 5 \& Title : group \& Usage : $embossprogram\->group \& Function: sets/gets the group of the EMBOSS program \& Setting is done by the EMBOSSFactory object, \& you should only get it. \& \& If the application is assigned into a subgroup \& use l to get it. \& Throws : \& Returns : string, group name \& Args : group string .Ve .SS "subgroup" .IX Subsection "subgroup" .Vb 8 \& Title : subgroup \& Usage : $embossprogram\->subgroup \& Function: sets/gets the subgroup of the EMBOSS program \& Setting is done by the EMBOSSFactory object, \& you should only get it. \& Throws : \& Returns : string, subgroup name; undef if not defined \& Args : None .Ve .SS "program_dir" .IX Subsection "program_dir" .Vb 6 \& Title : program_dir \& Usage : \& Function: Required by WrapperBase \& Throws : \& Returns : Name of directory with EMBOSS programs \& Args : .Ve .SS "program_path" .IX Subsection "program_path" .Vb 6 \& Title : program_path \& Usage : \& Function: Required by WrapperBase \& Throws : \& Returns : Full path of program \& Args : .Ve .SS "executable" .IX Subsection "executable" .Vb 6 \& Title : executable \& Usage : \& Function: Required by WrapperBase \& Throws : \& Returns : Name of program \& Args : .Ve