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.\" ========================================================================
.\"
.IX Title "Bio::Restriction::Enzyme::MultiCut 3pm"
.TH Bio::Restriction::Enzyme::MultiCut 3pm "2018-10-27" "perl v5.26.2" "User Contributed Perl Documentation"
.\" For nroff, turn off justification.  Always turn off hyphenation; it makes
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.SH "NAME"
Bio::Restriction::Enzyme::MultiCut \- A single restriction endonuclease
.SH "SYNOPSIS"
.IX Header "SYNOPSIS"
.Vb 3
\&  # set up a single restriction enzyme. This contains lots of
\&  # information about the enzyme that is generally parsed from a
\&  # rebase file and can then be read back
\&
\&  use Bio::Restriction::Enzyme;
.Ve
.SH "DESCRIPTION"
.IX Header "DESCRIPTION"
This module defines a restriction endonuclease class where one object
represents one of the distinct recognition sites for that enzyme. The
method others stores references to other objects with
alternative sites.
.PP
In this schema each object within an EnzymeCollection can be checked
for matching a sequence.
.PP
\&\s-1REBASE\s0 report notation \f(CW\*(C`Bsp24I (8/13)GACNNNNNNTGG(12/7)\*(C'\fR means:
.PP
.Vb 3
\&   Bsp24I
\&             5\*(Aq      ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^   3\*(Aq
\&             3\*(Aq ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^        5\*(Aq
.Ve
.SH "FEEDBACK"
.IX Header "FEEDBACK"
.SS "Mailing Lists"
.IX Subsection "Mailing Lists"
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
.PP
.Vb 2
\&  bioperl\-l@bioperl.org                  \- General discussion
\&  http://bioperl.org/wiki/Mailing_lists  \- About the mailing lists
.Ve
.SS "Support"
.IX Subsection "Support"
Please direct usage questions or support issues to the mailing list:
.PP
\&\fIbioperl\-l@bioperl.org\fR
.PP
rather than to the module maintainer directly. Many experienced and 
reponsive experts will be able look at the problem and quickly 
address it. Please include a thorough description of the problem 
with code and data examples if at all possible.
.SS "Reporting Bugs"
.IX Subsection "Reporting Bugs"
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
.PP
.Vb 1
\&  https://github.com/bioperl/bioperl\-live/issues
.Ve
.SH "AUTHOR"
.IX Header "AUTHOR"
Heikki Lehvaslaiho, heikki-at-bioperl-dot-org
.SH "CONTRIBUTORS"
.IX Header "CONTRIBUTORS"
Rob Edwards, redwards@utmem.edu
.SH "COPYRIGHT"
.IX Header "COPYRIGHT"
Copyright (c) 2003 Rob Edwards.
.PP
Some of this work is Copyright (c) 1997\-2002 Steve A. Chervitz. All
Rights Reserved.  This module is free software; you can redistribute
it and/or modify it under the same terms as Perl itself.
.SH "SEE ALSO"
.IX Header "SEE ALSO"
Bio::Restriction::Enzyme, Bio::Restriction::Analysis, 
Bio::Restriction::EnzymeCollection
.SH "APPENDIX"
.IX Header "APPENDIX"
Methods beginning with a leading underscore are considered private and
are intended for internal use by this module. They are not considered
part of the public interface and are described here for documentation
purposes only.
.SS "new"
.IX Subsection "new"
.Vb 5
\& Title     : new
\& Function
\& Function  : Initializes the enzyme object
\& Returns   : The Restriction::Enzyme::MultiCut object
\& Argument  :
.Ve
.SS "others"
.IX Subsection "others"
.Vb 5
\& Title     : others
\& Usage     : $re\->others(@enz_obj_array);
\& Function  : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
\& Arguments : optional array of Enzyme::MultiCut objects
\& Returns   : array of Enzyme objects
.Ve
.PP
Added for compatibility to \s-1REBASE\s0
.SS "purge_others"
.IX Subsection "purge_others"
.Vb 5
\& Title     : purge_others
\& Usage     : $re\->purge_references();
\& Function  : Purges the set of references for this enzyme
\& Arguments : 
\& Returns   :
.Ve