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Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" Bio::LiveSeq::Transcript \- Transcript class for LiveSeq .SH "SYNOPSIS" .IX Header "SYNOPSIS" .Vb 1 \& # documentation needed .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" This stores information about coding sequences (\s-1CDS\s0). The implementation is that a Transcript object accesses a collection of Exon objects, inferring from them the nucleotide structure and sequence. .SH "AUTHOR \- Joseph A.L. Insana" .IX Header "AUTHOR - Joseph A.L. Insana" Email: Insana@ebi.ac.uk, jinsana@gmx.net .SH "APPENDIX" .IX Header "APPENDIX" The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ .SS "new" .IX Subsection "new" .Vb 2 \& Title : new \& Usage : $transcript = Bio::LiveSeq::Transcript\->new(\-exons => \e@obj_refs); \& \& Function: generates a new Bio::LiveSeq::Transcript \& Returns : reference to a new object of class Transcript \& Errorcode \-1 \& Args : reference to an array of Exon object references .Ve .SS "all_Exons" .IX Subsection "all_Exons" .Vb 6 \& Title : all_Exons \& Usage : $transcript_obj\->all_Exons() \& Function: returns references to all Exon objects the Transcript is composed of \& Example : foreach $exon ($transcript\->all_Exons()) { do_something } \& Returns : array of object references \& Args : none .Ve .SS "downstream_seq" .IX Subsection "downstream_seq" .Vb 10 \& Title : downstream_seq \& Usage : $transcript_obj\->downstream_seq() \& : $transcript_obj\->downstream_seq(64) \& Function: returns a string of nucleotides downstream of the end of the \& CDS. If there is some information of the real mRNA, from features in \& an attached Gene object, it will return up to those boundaries. \& Otherwise it will return 1000 nucleotides. \& If an argument is given it will override the default 1000 number \& and return instead /that/ requested number of nucleotides. \& But if a Gene object is attached, this argument will be ignored. \& Returns : string \& Args : an optional integer number of nucleotides to be returned instead of \& the default if no gene attached .Ve .SS "upstream_seq" .IX Subsection "upstream_seq" .Vb 6 \& Title : upstream_seq \& Usage : $transcript_obj\->upstream_seq() \& : $transcript_obj\->upstream_seq(64) \& Function: just like downstream_seq but returns nucleotides before the ATG \& Note : the default, if no Gene information present and no nucleotides \& number given, is to return up to 400 nucleotides. .Ve .SS "get_Translation" .IX Subsection "get_Translation" .Vb 6 \& Title : valid \& Usage : $translation = $obj\->get_Translation() \& Function: retrieves the reference to the object of class Translation (if any) \& attached to a LiveSeq object \& Returns : object reference \& Args : none .Ve .SS "translation_table" .IX Subsection "translation_table" .Vb 7 \& Title : translation_table \& Usage : $name = $obj\->translation_table; \& : $name = $obj\->translation_table(11); \& Function: Returns or sets the translation_table used for translating the \& transcript. \& If it has never been set, it will return undef. \& Returns : an integer .Ve .SS "frame" .IX Subsection "frame" .Vb 11 \& Title : frame \& Usage : $frame = $transcript\->frame($label); \& Function: Returns the frame of a particular nucleotide. \& Frame can be 0 1 or 2 and means the position in the codon triplet \& of the particulat nucleotide. 0 is the first codon_position. \& Codon_position (1 2 3) is simply frame+1. \& If the label asked for is not inside the Transcript, \-1 will be \& returned. \& Args : a label \& Returns : 0 1 or 2 \& Errorcode \-1 .Ve