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Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" Bio::DB::SeqFeature::Store::DBI::mysql \-\- Mysql implementation of Bio::DB::SeqFeature::Store .SH "SYNOPSIS" .IX Header "SYNOPSIS" .Vb 1 \& use Bio::DB::SeqFeature::Store; \& \& # Open the sequence database \& my $db = Bio::DB::SeqFeature::Store\->new(\-adaptor => \*(AqDBI::mysql\*(Aq, \& \-dsn => \*(Aqdbi:mysql:test\*(Aq); \& \& # get a feature from somewhere \& my $feature = Bio::SeqFeature::Generic\->new(...); \& \& # store it \& $db\->store($feature) or die "Couldn\*(Aqt store!"; \& \& # primary ID of the feature is changed to indicate its primary ID \& # in the database... \& my $id = $feature\->primary_id; \& \& # get the feature back out \& my $f = $db\->fetch($id); \& \& # change the feature and update it \& $f\->start(100); \& $f\->update($f) or die "Couldn\*(Aqt update!"; \& \& # searching... \& # ...by id \& my @features = $db\->fetch_many(@list_of_ids); \& \& # ...by name \& @features = $db\->get_features_by_name(\*(AqZK909\*(Aq); \& \& # ...by alias \& @features = $db\->get_features_by_alias(\*(Aqsma\-3\*(Aq); \& \& # ...by type \& @features = $db\->get_features_by_name(\*(Aqgene\*(Aq); \& \& # ...by location \& @features = $db\->get_features_by_location(\-seq_id=>\*(AqChr1\*(Aq,\-start=>4000,\-end=>600000); \& \& # ...by attribute \& @features = $db\->get_features_by_attribute({description => \*(Aqprotein kinase\*(Aq}) \& \& # ...by the GFF "Note" field \& @result_list = $db\->search_notes(\*(Aqkinase\*(Aq); \& \& # ...by arbitrary combinations of selectors \& @features = $db\->features(\-name => $name, \& \-type => $types, \& \-seq_id => $seqid, \& \-start => $start, \& \-end => $end, \& \-attributes => $attributes); \& \& # ...using an iterator \& my $iterator = $db\->get_seq_stream(\-name => $name, \& \-type => $types, \& \-seq_id => $seqid, \& \-start => $start, \& \-end => $end, \& \-attributes => $attributes); \& \& while (my $feature = $iterator\->next_seq) { \& # do something with the feature \& } \& \& # ...limiting the search to a particular region \& my $segment = $db\->segment(\*(AqChr1\*(Aq,5000=>6000); \& my @features = $segment\->features(\-type=>[\*(AqmRNA\*(Aq,\*(Aqmatch\*(Aq]); \& \& # getting & storing sequence information \& # Warning: this returns a string, and not a PrimarySeq object \& $db\->insert_sequence(\*(AqChr1\*(Aq,\*(AqGATCCCCCGGGATTCCAAAA...\*(Aq); \& my $sequence = $db\->fetch_sequence(\*(AqChr1\*(Aq,5000=>6000); \& \& # what feature types are defined in the database? \& my @types = $db\->types; \& \& # create a new feature in the database \& my $feature = $db\->new_feature(\-primary_tag => \*(AqmRNA\*(Aq, \& \-seq_id => \*(Aqchr3\*(Aq, \& \-start => 10000, \& \-end => 11000); .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" Bio::DB::SeqFeature::Store::mysql is the Mysql adaptor for Bio::DB::SeqFeature::Store. You will not create it directly, but instead use Bio::DB::SeqFeature::Store\->\fInew()\fR to do so. .PP See Bio::DB::SeqFeature::Store for complete usage instructions. .SS "Using the Mysql adaptor" .IX Subsection "Using the Mysql adaptor" Before you can use the adaptor, you must use the mysqladmin tool to create a database and establish a user account with write permission. In order to use \*(L"fast\*(R" loading, the user account must have \&\*(L"file\*(R" privileges. .PP To establish a connection to the database, call Bio::DB::SeqFeature::Store\->new(\-adaptor=>'DBI::mysql',@more_args). The additional arguments are as follows: .PP .Vb 2 \& Argument name Description \& \-\-\-\-\-\-\-\-\-\-\-\-\- \-\-\-\-\-\-\-\-\-\-\- \& \& \-dsn The database name. You can abbreviate \& "dbi:mysql:foo" as "foo" if you wish. \& \& \-user Username for authentication. \& \& \-pass Password for authentication. \& \& \-namespace A prefix to attach to each table. This allows you \& to have several virtual databases in the same \& physical database. \& \& \-temp Boolean flag. If true, a temporary database \& will be created and destroyed as soon as \& the Store object goes out of scope. (synonym \-temporary) \& \& \-autoindex Boolean flag. If true, features in the database will be \& reindexed every time they change. This is the default. \& \& \& \-tmpdir Directory in which to place temporary files during "fast" loading. \& Defaults to File::Spec\->tmpdir(). (synonyms \-dump_dir, \-dumpdir, \-tmp) \& \& \-dbi_options A hashref to pass to DBI\->connect\*(Aqs 4th argument, the "attributes." \& (synonyms \-options, \-dbi_attr) \& \& \-write Pass true to open database for writing or updating. .Ve .PP If successful, a new instance of Bio::DB::SeqFeature::Store::DBI::mysql will be returned. .PP In addition to the standard methods supported by all well-behaved Bio::DB::SeqFeature::Store databases, several following adaptor-specific methods are provided. These are described in the next sections. .SS "types" .IX Subsection "types" .Vb 6 \& Title : types \& Usage : @type_list = $db\->types \& Function: Get all the types in the database \& Returns : array of Bio::DB::GFF::Typename objects \& Args : none \& Status : public .Ve .SS "toplevel_types" .IX Subsection "toplevel_types" .Vb 6 \& Title : toplevel_types \& Usage : @type_list = $db\->toplevel_types \& Function: Get the toplevel types in the database \& Returns : array of Bio::DB::GFF::Typename objects \& Args : none \& Status : public .Ve .PP This is similar to \fItypes()\fR but only returns the types of \&\s-1INDEXED\s0 (toplevel) features.