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Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" Bio::DB::GenPept \- Database object interface to GenPept .SH "SYNOPSIS" .IX Header "SYNOPSIS" .Vb 1 \& $gb = Bio::DB::GenPept\->new(); \& \& $seq = $gb\->get_Seq_by_id(\*(Aq195055\*(Aq); # Unique ID \& \& # or ... \& \& $seq = $gb\->get_Seq_by_acc(\*(AqDEECTH\*(Aq); # Accession Number \& \& my $seqio = $gb\->get_Stream_by_id([\*(Aq195055\*(Aq, \*(AqDEECTH\*(Aq]); \& while( my $seq = $seqio\->next_seq ) { \& print "seq is is ", $seq\->display_id, "\en"; \& } .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" Allows the dynamic retrieval of Sequence objects (Bio::Seq) from the GenPept database at \s-1NCBI,\s0 via an Entrez query. .PP \&\s-1WARNING:\s0 Please do \s-1NOT\s0 spam the Entrez web server with multiple requests. \&\s-1NCBI\s0 offers Batch Entrez for this purpose. Batch Entrez support will likely be supported in a future version of DB::GenPept. .PP Currently the only return format supported by \s-1NCBI\s0 Entrez for GenPept database is GenPept format, so any format specification passed to GenPept will be ignored still be forced to GenPept format (which is just GenBank format). .SH "FEEDBACK" .IX Header "FEEDBACK" .SS "Mailing Lists" .IX Subsection "Mailing Lists" User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. .PP .Vb 2 \& bioperl\-l@bioperl.org \- General discussion \& http://bioperl.org/wiki/Mailing_lists \- About the mailing lists .Ve .SS "Support" .IX Subsection "Support" Please direct usage questions or support issues to the mailing list: .PP \&\fIbioperl\-l@bioperl.org\fR .PP rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. .SS "Reporting Bugs" .IX Subsection "Reporting Bugs" Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: .PP .Vb 1 \& https://github.com/bioperl/bioperl\-live/issues .Ve .SH "AUTHOR \- Aaron Mackey, Jason Stajich" .IX Header "AUTHOR - Aaron Mackey, Jason Stajich" Email amackey@virginia.edu Email jason@bioperl.org .SH "APPENDIX" .IX Header "APPENDIX" The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ .SS "get_params" .IX Subsection "get_params" .Vb 6 \& Title : get_params \& Usage : my %params = $self\->get_params($mode) \& Function: Returns key,value pairs to be passed to NCBI database \& for either \*(Aqbatch\*(Aq or \*(Aqsingle\*(Aq sequence retrieval method \& Returns : a key,value pair hash \& Args : \*(Aqsingle\*(Aq or \*(Aqbatch\*(Aq mode for retrieval .Ve .SS "default_format" .IX Subsection "default_format" .Vb 5 \& Title : default_format \& Usage : my $format = $self\->default_format \& Function: Returns default sequence format for this module \& Returns : string \& Args : none .Ve .SH "Routines from Bio::DB::WebDBSeqI and Bio::DB::RandomAccessI" .IX Header "Routines from Bio::DB::WebDBSeqI and Bio::DB::RandomAccessI" .SS "get_Seq_by_id" .IX Subsection "get_Seq_by_id" .Vb 6 \& Title : get_Seq_by_id \& Usage : $seq = $db\->get_Seq_by_id(\*(AqROA1_HUMAN\*(Aq) \& Function: Gets a Bio::Seq object by its name \& Returns : a Bio::Seq object \& Args : the id (as a string) of a sequence \& Throws : "id does not exist" exception .Ve .SS "get_Seq_by_acc" .IX Subsection "get_Seq_by_acc" .Vb 5 \& Title : get_Seq_by_acc \& Usage : $seq = $db\->get_Seq_by_acc(\*(AqAAC73346\*(Aq); \& Function: Gets a Seq objects by accession number \& Returns : Bio::Seq object \& Args : accession number to retrieve by .Ve .SH "Routines implemented by Bio::DB::NCBIHelper" .IX Header "Routines implemented by Bio::DB::NCBIHelper" .SS "get_request" .IX Subsection "get_request" .Vb 5 \& Title : get_request \& Usage : my $url = $self\->get_request \& Function: HTTP::Request \& Returns : \& Args : %qualifiers = a hash of qualifiers (ids, format, etc) .Ve .SS "get_Stream_by_id" .IX Subsection "get_Stream_by_id" .Vb 6 \& Title : get_Stream_by_id \& Usage : $stream = $db\->get_Stream_by_id( [$uid1, $uid2] ); \& Function: Gets a series of Seq objects by unique identifiers \& Returns : a Bio::SeqIO stream object \& Args : $ref : a reference to an array of unique identifiers for \& the desired sequence entries .Ve .SS "get_Stream_by_acc (2)" .IX Subsection "get_Stream_by_acc (2)" .Vb 7 \& Title : get_Stream_by_acc \& Usage : $seq = $db\->get_Stream_by_acc($acc); \& Function: Gets a series of Seq objects by accession numbers \& Returns : a Bio::SeqIO stream object \& Args : $ref : a reference to an array of accession numbers for \& the desired sequence entries \& Note : For GenBank, this just calls the same code for get_Stream_by_id() .Ve .SS "request_format" .IX Subsection "request_format" .Vb 6 \& Title : request_format \& Usage : my $format = $self\->request_format; \& $self\->request_format($format); \& Function: Get/Set sequence format retrieval \& Returns : string representing format \& Args : $format = sequence format .Ve