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Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" Bio::DB::EntrezGene \- Database object interface to Entrez Gene .SH "SYNOPSIS" .IX Header "SYNOPSIS" .Vb 1 \& use Bio::DB::EntrezGene; \& \& my $db = Bio::DB::EntrezGene\->new; \& \& my $seq = $db\->get_Seq_by_id(2); # Gene id \& \& # or ... \& \& my $seqio = $db\->get_Stream_by_id([2, 4693, 3064]); # Gene ids \& while ( my $seq = $seqio\->next_seq ) { \& print "id is ", $seq\->display_id, "\en"; \& } .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" Allows the dynamic retrieval of Sequence objects from the Entrez Gene database at \s-1NCBI,\s0 via an Entrez query using Gene ids. .PP This module requires the \s-1CPAN\s0 Bio::ASN1 module. .PP \&\s-1WARNING:\s0 Please do \s-1NOT\s0 spam the Entrez web server with multiple requests. \&\s-1NCBI\s0 offers Batch Entrez for this purpose. .SH "NOTES" .IX Header "NOTES" The Entrez eutils \s-1API\s0 does not allow Entrez Gene queries by name as of this writing, therefore there are only get_Seq_by_id and get_Stream_by_id methods in this module, and these expect Gene ids. There are no get_Seq_by_acc or get_Stream_by_acc methods. .SH "FEEDBACK" .IX Header "FEEDBACK" .SS "Mailing Lists" .IX Subsection "Mailing Lists" User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. .PP .Vb 2 \& bioperl\-l@bioperl.org \- General discussion \& http://bioperl.org/wiki/Mailing_lists \- About the mailing lists .Ve .SS "Support" .IX Subsection "Support" Please direct usage questions or support issues to the mailing list: .PP \&\fIbioperl\-l@bioperl.org\fR .PP rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. .SS "Reporting Bugs" .IX Subsection "Reporting Bugs" Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: .PP .Vb 1 \& https://github.com/bioperl/bioperl\-live/issues .Ve .SH "AUTHOR \- Brian Osborne" .IX Header "AUTHOR - Brian Osborne" Email bosborne at alum.mit.edu .SH "APPENDIX" .IX Header "APPENDIX" The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ .SS "get_params" .IX Subsection "get_params" .Vb 6 \& Title : get_params \& Usage : my %params = $self\->get_params($mode) \& Function: Returns key,value pairs to be passed to NCBI database \& for either \*(Aqbatch\*(Aq or \*(Aqsingle\*(Aq sequence retrieval method \& Returns : A key,value pair hash \& Args : \*(Aqsingle\*(Aq or \*(Aqbatch\*(Aq mode for retrieval .Ve .SS "default_format" .IX Subsection "default_format" .Vb 5 \& Title : default_format \& Usage : my $format = $self\->default_format \& Function: Returns default sequence format for this module \& Returns : string \& Args : none .Ve .SH "Routines from Bio::DB::WebDBSeqI and Bio::DB::RandomAccessI" .IX Header "Routines from Bio::DB::WebDBSeqI and Bio::DB::RandomAccessI" .SS "get_Seq_by_id" .IX Subsection "get_Seq_by_id" .Vb 6 \& Title : get_Seq_by_id \& Usage : $seq = $db\->get_Seq_by_id(2) \& Function: Gets a Bio::Seq object by its name \& Returns : A Bio::Seq object \& Args : Gene id \& Throws : "id does not exist" exception .Ve .SH "Routines implemented by Bio::DB::NCBIHelper" .IX Header "Routines implemented by Bio::DB::NCBIHelper" .SS "get_request" .IX Subsection "get_request" .Vb 5 \& Title : get_request \& Usage : my $url = $self\->get_request \& Function: HTTP::Request \& Returns : \& Args : %qualifiers = a hash of qualifiers (ids, format, etc) .Ve .SS "get_Stream_by_id" .IX Subsection "get_Stream_by_id" .Vb 5 \& Title : get_Stream_by_id \& Usage : $stream = $db\->get_Stream_by_id( [$gid1, $gid2] ); \& Function: Gets a series of Seq objects using Gene ids \& Returns : A Bio::SeqIO stream object \& Args : A reference to an array of Gene ids .Ve .SS "request_format" .IX Subsection "request_format" .Vb 6 \& Title : request_format \& Usage : my $format = $self\->request_format; \& $self\->request_format($format); \& Function: Get or set sequence format retrieval \& Returns : String representing format \& Args : $format = sequence format .Ve