.\" -*- mode: troff; coding: utf-8 -*- .\" Automatically generated by Pod::Man 5.01 (Pod::Simple 3.43) .\" .\" Standard preamble: .\" ======================================================================== .de Sp \" Vertical space (when we can't use .PP) .if t .sp .5v .if n .sp .. .de Vb \" Begin verbatim text .ft CW .nf .ne \\$1 .. .de Ve \" End verbatim text .ft R .fi .. .\" \*(C` and \*(C' are quotes in nroff, nothing in troff, for use with C<>. .ie n \{\ . ds C` "" . ds C' "" 'br\} .el\{\ . ds C` . ds C' 'br\} .\" .\" Escape single quotes in literal strings from groff's Unicode transform. .ie \n(.g .ds Aq \(aq .el .ds Aq ' .\" .\" If the F register is >0, we'll generate index entries on stderr for .\" titles (.TH), headers (.SH), subsections (.SS), items (.Ip), and index .\" entries marked with X<> in POD. Of course, you'll have to process the .\" output yourself in some meaningful fashion. .\" .\" Avoid warning from groff about undefined register 'F'. .de IX .. .nr rF 0 .if \n(.g .if rF .nr rF 1 .if (\n(rF:(\n(.g==0)) \{\ . if \nF \{\ . de IX . tm Index:\\$1\t\\n%\t"\\$2" .. . if !\nF==2 \{\ . nr % 0 . nr F 2 . \} . \} .\} .rr rF .\" ======================================================================== .\" .IX Title "Bio::Tradis::Parser::Bam 3pm" .TH Bio::Tradis::Parser::Bam 3pm 2024-01-25 "perl v5.38.2" "User Contributed Perl Documentation" .\" For nroff, turn off justification. Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH NAME Bio::Tradis::Parser::Bam \- Very basic BAM parser. Limited functionality. .SH VERSION .IX Header "VERSION" version 1.4.5 .SH SYNOPSIS .IX Header "SYNOPSIS" Parses BAM files and gives access to basic info in them. .PP .Vb 1 \& use Bio::Tradis::Parser::Bam; \& \& my $pipeline = Bio::Tradis::Parser::Bam\->new(file => \*(Aqabc\*(Aq); \& $pipeline\->read_info; \& $pipeline\->next_read; \& $pipeline\->seq_info; \& $pipeline\->is_mapped; \& $pipeline\->is_reverse; .Ve .PP =seq_info Reads BAM header and returns a hash (keys are sequence ids, values are hash refs with keys as tags (like LN and M5)) .PP =next_read Moves _currentread to the next entry in the BAM. Returns 0 if EOF. .PP =read_info Returns info from _currentread = hash reference with field name as key. Standard fields are named as per the SAM format specification: 1 : QNAME 2 : FLAG 3 : RNAME 4 : POS 5 : MAPQ 6 : CIGAR 7 : RNEXT 8 : PNEXT 9 : TLEN 10 : SEQ 11 : QUAL Additional fields will use their tag names. Complete line is returned with key READ .PP =is_mapped Parses the flag for the current read and determines if mapped. Returns 0 or 1. .PP =is_reverse Parses the flag for the current read and determines if reverse complemented. Returns 0 or 1. .SH AUTHOR .IX Header "AUTHOR" Carla Cummins .SH "COPYRIGHT AND LICENSE" .IX Header "COPYRIGHT AND LICENSE" This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. .PP This is free software, licensed under: .PP .Vb 1 \& The GNU General Public License, Version 3, June 2007 .Ve