'\" t
.\"     Title: rnaspades
.\"    Author: [see the "AUTHORS" section]
.\" Generator: Asciidoctor 1.5.6.2
.\"      Date: 
.\"    Manual: \ \&
.\"    Source: \ \&
.\"  Language: English
.\"
.TH "RNASPADES" "1" "" "\ \&" "\ \&"
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.SH "NAME"
rnaspades \- SPAdes genome assembler (rnaSPAdes mode)
.SH "SYNOPSIS"
.sp
\fBrnaspades\fP [options] \-o <output_dir>
.SH "DESCRIPTION"
.sp
\fBrnaspades\fP runs \fBSPAdes\fP in rnaSPAdes mode.
.SH "OPTIONS"
.SS "Basic options"
.sp
\fB\-o\fP <output_dir>
.RS 4
directory to store all the resulting files (required)
.RE
.sp
\fB\-\-iontorrent\fP
.RS 4
this flag is required for IonTorrent data
.RE
.sp
\fB\-\-test\fP
.RS 4
runs SPAdes on toy dataset
.RE
.sp
\fB\-h\fP/\fB\-\-help\fP
.RS 4
prints this usage message
.RE
.SS "Input data"
.sp
\fB\-\-12\fP <filename>
.RS 4
file with interlaced forward and reverse paired\-end reads
.RE
.sp
\fB\-1\fP <filename>
.RS 4
file with forward paired\-end reads
.RE
.sp
\fB\-2\fP <filename>
.RS 4
file with reverse paired\-end reads
.RE
.sp
\fB\-s\fP <filename>
.RS 4
file with unpaired reads
.RE
.sp
\fB\-\-pe<#>\-12\fP <filename>
.RS 4
file with interlaced reads for paired\-end library number <#> (<#> = 1,2,3,4,5)
.RE
.sp
\fB\-\-pe<#>\-1\fP <filename>
.RS 4
file with forward reads for paired\-end library number <#> (<#> = 1,2,3,4,5)
.RE
.sp
\fB\-\-pe<#>\-2\fP <filename>
.RS 4
file with reverse reads for paired\-end library number <#> (<#> = 1,2,3,4,5)
.RE
.sp
\fB\-\-pe<#>\-s\fP <filename>
.RS 4
file with unpaired reads for paired\-end library number <#> (<#> = 1,2,3,4,5)
.RE
.sp
\fB\-\-pe<#>\-<or>\fP
.RS 4
orientation of reads for paired\-end library number <#> (<#> = 1,2,3,4,5; <or> = fr, rf, ff)
.RE
.sp
\fB\-\-s<#>\fP <filename>
.RS 4
file with unpaired reads for single reads library number <#> (<#> = 1,2,3,4,5)
.RE
.sp
\fB\-\-trusted\-contigs\fP <filename>
.RS 4
file with trusted contigs
.RE
.sp
\fB\-\-untrusted\-contigs\fP <filename>
.RS 4
file with untrusted contigs
.RE
.SS "Pipeline options"
.sp
\fB\-\-only\-error\-correction\fP
.RS 4
runs only read error correction (without assembling)
.RE
.sp
\fB\-\-only\-assembler\fP
.RS 4
runs only assembling (without read error correction)
.RE
.sp
\fB\-\-continue\fP
.RS 4
continue run from the last available check\-point
.RE
.sp
\fB\-\-restart\-from\fP <cp>
.RS 4
restart run with updated options and from the specified check\-point (\(aqec\(aq, \(aqas\(aq, \(aqk<int>\(aq, \(aqmc\(aq)
.RE
.sp
\fB\-\-disable\-gzip\-output\fP
.RS 4
forces error correction not to compress the corrected reads
.RE
.sp
\fB\-\-disable\-rr\fP
.RS 4
disables repeat resolution stage of assembling
.RE
.SS "Advanced options"
.sp
\fB\-\-dataset\fP <filename>
.RS 4
file with dataset description in YAML format
.RE
.sp
\fB\-t\fP/\fB\-\-threads\fP <int>
.RS 4
number of threads [default: 16]
.RE
.sp
\fB\-m\fP/\fB\-\-memory\fP <int>
.RS 4
RAM limit for SPAdes in Gb (terminates if exceeded) [default: 250]
.RE
.sp
\fB\-\-tmp\-dir\fP <dirname>
.RS 4
directory for temporary files [default: <output_dir>/tmp]
.RE
.sp
\fB\-k\fP <int,int,...>
.RS 4
comma\-separated list of k\-mer sizes (must be odd and less than 128) [default: \(aqauto\(aq]
.RE
.sp
\fB\-\-cov\-cutoff\fP <float>
.RS 4
coverage cutoff value (a positive float number, or \(aqauto\(aq, or \(aqoff\(aq) [default: \(aqoff\(aq]
.RE
.sp
\fB\-\-phred\-offset\fP <33 or 64>
.RS 4
PHRED quality offset in the input reads (33 or 64) [default: auto\-detect]
.RE