.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.41.1. .TH FILL-FS "1" "February 2013" "fill-fs" "User Commands" .SH NAME fill-fs \- Annotate VCF with flanking sequence .SH SYNOPSIS .B fill-fs [\fIOPTIONS\fR] \fIfile.vcf\fR .SH DESCRIPTION About: Annotate VCF with flanking sequence (INFO/FS tag) .SH OPTIONS .TP \fB\-b\fR, \fB\-\-bed\-mask\fR Regions to mask (tabix indexed), multiple files can be given .TP \fB\-c\fR, \fB\-\-cluster\fR Do self\-masking of clustered variants within this range. .TP \fB\-l\fR, \fB\-\-length\fR Flanking sequence length [100] .TP \fB\-m\fR, \fB\-\-mask\-char\fR The character to use or "lc" for lowercase. This option must preceed \fB\-b\fR, \fB\-v\fR or \fB\-c\fR in order to take effect. With multiple files works .IP as a switch on the command line, see the example below [N] .TP \fB\-r\fR, \fB\-\-refseq\fR The reference sequence. .TP \fB\-v\fR, \fB\-\-vcf\-mask\fR Mask known variants in the flanking sequence, multiple files can be given (tabix indexed) .TP \fB\-h\fR, \-?, \fB\-\-help\fR This help message. .SS "Example:" .IP # Mask variants from the VCF file with N's and use lowercase for the bed file regions fill\-fs file.vcf \fB\-v\fR mask.vcf \fB\-m\fR lc \fB\-b\fR mask.bed .IP