.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.43.3. .TH MIRABAIT: "1" "August 2013" "MIRALIB version 3.9.18" "User Commands" .SH NAME mirabait \- select reads from a read collection .SH SYNOPSIS .B mirabait [\fI\-f \fR] [\fI\-t [\-t ...]\fR] [\fI\-iklor\fR] baitfile infile .SH DESCRIPTION \fBmirabait\fR selects reads from a read collection which are partly similar or equal to sequences defined as target baits. Similarity is defined by finding a user-adjustable number of common k-mers (sequences of k consecutive bases) which are the same in the bait sequences and the screened sequences to be selected, either in forward or reverse complement direction. .SH OPTIONS .TP \fB\-f\fR load this type of project files, where fromtype is: .TP caf sequences from CAF .TP maf sequences from MAF .TP phd sequences from a PHD .TP gbf sequences from a GBF .TP fasta sequences from a FASTA .TP fastq sequences from a FASTQ .TP \fB\-t\fR write the sequences to this type (multiple mentions of \fB\-t\fR are allowed): .TP fasta sequences to FASTA .TP fastq sequences to FASTQ .TP caf sequences to CAF .TP maf sequences to MAF .TP txt sequence names to text file .TP \fB\-k\fR k\-mer, length of bait in bases (<32, default=31) .TP \fB\-n\fR Min. number of k\-mer baits needed (default=1) .TP \fB\-i\fR Inverse hit: writes only sequences that do not hit bait .TP \fB\-r\fR No checking of reverse complement direction .TP \fB\-o\fR fastq quality Offset (only for \fB\-f\fR = 'fastq') Offset of quality values in FASTQ file. Default: 33 A value of 0 tries to automatically recognise. .TP \fB\-f\fR load this type of project files, where fromtype is: .TP caf sequences from CAF .TP maf sequences from MAF .TP phd sequences from a PHD .TP gbf sequences from a GBF .TP fasta sequences from a FASTA .TP fastq sequences from a FASTQ .TP \fB\-t\fR write the sequences to this type (multiple mentions of \fB\-t\fR are allowed): .TP fasta sequences to FASTA .TP fastq sequences to FASTQ .TP caf sequences to CAF .TP maf sequences to MAF .TP txt sequence names to text file .TP \fB\-k\fR k\-mer, length of bait in bases (<32, default=31) .TP \fB\-n\fR Min. number of k\-mer baits needed (default=1) .TP \fB\-i\fR Inverse hit: writes only sequences that do not hit bait .TP \fB\-r\fR No checking of reverse complement direction .TP \fB\-o\fR fastq quality Offset (only for \fB\-f\fR = 'fastq') Offset of quality values in FASTQ file. Default: 33 A value of 0 tries to automatically recognise. .SH AUTHOR Bastien Chevreux (bach@chevreux.org)