.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.45.1. .TH VELVETH "1" "July 2014" "velveth 1.2.10+dfsg1" "User Commands" .SH NAME velveth \- simple hashing program .SH DESCRIPTION velveth \- simple hashing program Version 1.2.09 .SH OPTIONS .TP \fB\-strand_specific\fR : for strand specific transcriptome sequencing data (default: off) .TP \fB\-reuse_Sequences\fR : reuse Sequences file (or link) already in directory (no need to provide original filenames in this case (default: off) .TP \fB\-reuse_binary\fR : reuse binary sequences file (or link) already in directory (no need to provide original filenames in this case (default: off) .TP \fB\-noHash\fR : simply prepare Sequences file, do not hash reads or prepare Roadmaps file (default: off) .TP \fB\-create_binary\fR : create binary CnyUnifiedSeq file (default: off) .PP Synopsis: .SS "- Short single end reads:" .IP velveth Assem 29 \fB\-short\fR \fB\-fastq\fR s_1_sequence.txt .SS "- Paired-end short reads (remember to interleave paired reads):" .IP velveth Assem 31 \fB\-shortPaired\fR \fB\-fasta\fR interleaved.fna .PP \- Paired\-end short reads (using separate files for the paired reads) .IP velveth Assem 31 \fB\-shortPaired\fR \fB\-fasta\fR \fB\-separate\fR left.fa right.fa .SS "- Two channels and some long reads:" .IP velveth Assem 43 \fB\-short\fR \fB\-fastq\fR unmapped.fna \fB\-longPaired\fR \fB\-fasta\fR SangerReads.fasta .SS "- Three channels:" .IP velveth Assem 35 \fB\-shortPaired\fR \fB\-fasta\fR pe_lib1.fasta \fB\-shortPaired2\fR pe_lib2.fasta \fB\-short3\fR se_lib1.fa .SS "Output:" .IP directory/Roadmaps directory/Sequences .IP [Both files are picked up by graph, so please leave them there] .SH COPYRIGHT Copyright 2007, 2008 Daniel Zerbino (zerbino@ebi.ac.uk) .br This is free software; see the source for copying conditions. There is NO warranty; not even for MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. .PP Compilation settings: CATEGORIES = 2 MAXKMERLENGTH = 31 .PP Usage: \&./velveth directory hash_length {[\-file_format][\-read_type][\-separate|\-interleaved] filename1 [filename2 ...]} {...} [options] .TP directory : directory name for output files .TP hash_length : EITHER an odd integer (if even, it will be decremented) <= 31 (if above, will be reduced) : OR: m,M,s where m and M are odd integers (if not, they will be decremented) with m < M <= 31 (if above, will be reduced) .IP and s is a step (even number). Velvet will then hash from k=m to k=M with a step of s .TP filename : path to sequence file or \- for standard input .SS "File format options:" .HP \fB\-fasta\fR \fB\-fastq\fR \fB\-raw\fR \fB\-fasta\fR.gz \fB\-fastq\fR.gz \fB\-raw\fR.gz \fB\-sam\fR \fB\-bam\fR \fB\-fmtAuto\fR .IP (Note: \fB\-fmtAuto\fR will detect fasta or fastq, and will try the following programs for decompression : gunzip, pbunzip2, bunzip2 .SS "File layout options for paired reads (only for fasta and fastq formats):" .TP \fB\-interleaved\fR : File contains paired reads interleaved in the one file (default) .TP \fB\-separate\fR : Read 2 separate files for paired reads .SS "Read type options:" .HP \fB\-short\fR \fB\-shortPaired\fR .HP \fB\-short2\fR \fB\-shortPaired2\fR .HP \fB\-long\fR \fB\-longPaired\fR .HP \fB\-reference\fR