'\" t .\" Title: reapr-smaltmap .\" Author: [see the "AUTHOR(S)" section] .\" Generator: Asciidoctor 1.5.7.1 .\" Date: .\" Manual: \ \& .\" Source: \ \& .\" Language: English .\" .TH "REAPR\-SMALTMAP" "1" "" "\ \&" "\ \&" .ie \n(.g .ds Aq \(aq .el .ds Aq ' .ss \n[.ss] 0 .nh .ad l .de URL \fI\\$2\fP <\\$1>\\$3 .. .als MTO URL .if \n[.g] \{\ . mso www.tmac . am URL . ad l . . . am MTO . ad l . . . LINKSTYLE blue R < > .\} .SH "NAME" reapr\-smaltmap \- map read pairs using SMALT .SH "SYNOPSIS" .sp \fBreapr smaltmap\fP [options] .SH "DESCRIPTION" .sp Maps read pairs to an assembly with SMALT, making a final BAM file that is sorted, indexed and has duplicates removed. .sp The n\-th read in should be the mate of the n\-th read in . .sp It is assumed that reads are \(aqinnies\(aq, i.e. the correct orientation is reads in a pair pointing towards each other (\-\-\(-> \(<-\-\-). .SH "OPTIONS" .sp \fB\-k\fP .RS 4 The \-k option (kmer hash length) when indexing the genome with \(aqsmalt index\(aq [13] .RE .sp \fB\-s\fP .RS 4 The \-s option (step length) when indexing the genome with \(aqsmalt index\(aq [2] .RE .sp \fB\-m\fP .RS 4 The \-m option when mapping reads with \(aqsmalt map\(aq [not used by default] .RE .sp \fB\-n\fP .RS 4 The number of threads used when running \(aqsmalt map\(aq [1] .RE .sp \fB\-y\fP .RS 4 The \-y option when mapping reads with \(aqsmalt map\(aq. The default of 0.5 means that at least 50% of each read must map perfectly. Depending on the quality of your reads, you may want to increase this to be more stringent (or consider using \-m) [0.5] .RE .sp \fB\-x\fP .RS 4 Use this to just print the commands that will be run, instead of actually running them .RE .sp \fB\-u\fP .RS 4 The \-u option of \(aqsmalt sample\(aq. This is used to estimate the insert size from a sample of the reads, by mapping every n^th read pair [1000] .RE .SH "SEE ALSO" .sp reapr(1)