NAME¶
perlprimer - graphically specify amplicon of DNA or mRNA sequences and design
primers
DESCRIPTION¶
PerlPrimer calculates primer melting temperature using J. SantaLucia's
extensive nearest-neighbour thermodynamic parameters. To adjust for the salt
conditions of the PCR, PerlPrimer uses the empirical formula derived by von
Ahsen, et al. (2001) and allows the user to specify the concentration of Mg2+,
dNTPs and primers, or use standard PCR conditions. The result is a highly
accurate prediction of primer melting temperature, giving rise to a maximum
yield of product when amplified. PerlPrimer is written in Perl and requires
Perl/Tk. In addition, for QPCR functionality PerlPrimer requires the
open-source Spidey executable from NCBI. The program is designed to be
cross-platform compatible and has been developed and tested on both Microsoft
Windows and GNU/Linux-based operating systems. Users have also reported
success using the program under Mac OS X.
AUTHOR¶
perlprimer was written by Owen Marshall <owenjm@users.sourceforge.net>
This manual page was assembled by Steffen Moeller
<steffen_moeller@gmx.de>, for the Debian project (but may be used by
others).