.\" Automatically generated by Pod::Man 4.10 (Pod::Simple 3.35) .\" .\" Standard preamble: .\" ======================================================================== .de Sp \" Vertical space (when we can't use .PP) .if t .sp .5v .if n .sp .. .de Vb \" Begin verbatim text .ft CW .nf .ne \\$1 .. .de Ve \" End verbatim text .ft R .fi .. .\" Set up some character translations and predefined strings. \*(-- will .\" give an unbreakable dash, \*(PI will give pi, \*(L" will give a left .\" double quote, and \*(R" will give a right double quote. \*(C+ will .\" give a nicer C++. Capital omega is used to do unbreakable dashes and .\" therefore won't be available. \*(C` and \*(C' expand to `' in nroff, .\" nothing in troff, for use with C<>. .tr \(*W- .ds C+ C\v'-.1v'\h'-1p'\s-2+\h'-1p'+\s0\v'.1v'\h'-1p' .ie n \{\ . ds -- \(*W- . ds PI pi . if (\n(.H=4u)&(1m=24u) .ds -- \(*W\h'-12u'\(*W\h'-12u'-\" diablo 10 pitch . if (\n(.H=4u)&(1m=20u) .ds -- \(*W\h'-12u'\(*W\h'-8u'-\" diablo 12 pitch . ds L" "" . ds R" "" . ds C` "" . ds C' "" 'br\} .el\{\ . ds -- \|\(em\| . ds PI \(*p . ds L" `` . ds R" '' . ds C` . ds C' 'br\} .\" .\" Escape single quotes in literal strings from groff's Unicode transform. .ie \n(.g .ds Aq \(aq .el .ds Aq ' .\" .\" If the F register is >0, we'll generate index entries on stderr for .\" titles (.TH), headers (.SH), subsections (.SS), items (.Ip), and index .\" entries marked with X<> in POD. Of course, you'll have to process the .\" output yourself in some meaningful fashion. .\" .\" Avoid warning from groff about undefined register 'F'. .de IX .. .nr rF 0 .if \n(.g .if rF .nr rF 1 .if (\n(rF:(\n(.g==0)) \{\ . if \nF \{\ . de IX . tm Index:\\$1\t\\n%\t"\\$2" .. . if !\nF==2 \{\ . nr % 0 . nr F 2 . \} . \} .\} .rr rF .\" ======================================================================== .\" .IX Title "TFBS::PatternGen::Elph 3pm" .TH TFBS::PatternGen::Elph 3pm "2018-11-02" "perl v5.28.0" "User Contributed Perl Documentation" .\" For nroff, turn off justification. Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" TFBS::PatternGen::Elph \- a pattern factory that uses the Elph program .SH "SYNOPSIS" .IX Header "SYNOPSIS" .Vb 5 \& my $patterngen = \& TFBS::PatternGen::Elph\->new(\-seq_file=>\*(Aqsequences.fa\*(Aq, \& \-binary => \*(Aq/Elph/elph\*(Aq \& \-motif_length => [8, 9, 10], \& \-additional_params => \*(Aq\-x \-r \-e\*(Aq); \& \& my $pfm = $patterngen\->pattern(); # $pfm is now a TFBS::Matrix::PFM object .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" TFBS::PatternGen::Gibbs builds position frequency matrices using an advanced Gibbs sampling algorithm implemented in external \&\fIGibbs\fR program by Chip Lawrence. The algorithm can produce multiple patterns from a single set of sequences. .SS "new" .IX Subsection "new" .Vb 10 \& Title : new \& Usage : my $db = TFBS::PatternGen::Gibbs\->new(%args); \& Function: the constructor for the TFBS::PatternGen::Gibbs object \& Returns : a TFBS::PatternGen::Gibbs object \& Args : This method takes named arguments; \& you must specify one of the following three \& \-seq_list # a reference to an array of strings \& # and/or Bio::Seq objects \& # or \& \-seq_stream # A Bio::SeqIO object \& # or \& \-seq_file # the name of the fasta file containing \& # all the sequences \& Other arguments are: \& \-binary # a fully qualified path to Gibbs executable \& # OPTIONAL: default \*(AqGibbs\*(Aq \& \-nr_hits # a presumed number of pattern occurrences in the \& # sequence set: it can be a single integer, e.g. \& # \-nr_hits => 24 , or a reference to an array of \& # integers, e.g \-nr_hits => [12, 24, 36] \& \-motif_length # an expected length of motif in nucleotides: \& # it can be a single integer, e.g. \& # \-motif_length => 8 , or a reference to an \& # array ofintegers, e.g \-motif_length => [8..12] \& \-additional_params # a string containing additional \& # command\-line switches for the \& # Gibbs program .Ve .SS "pattern" .IX Subsection "pattern" .SS "all_patterns" .IX Subsection "all_patterns" .SS "patternSet" .IX Subsection "patternSet" The three methods listed above are used for the retrieval of patterns, and are common to all TFBS::PatternGen::* classes. Please see TFBS::PatternGen for details.