.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.6. .TH CODONW "1" "July 2018" "codonw 1.4.4" "User Commands" .SH NAME codonw \- Correspondence Analysis of Codon Usage .SH DESCRIPTION codonW [inputfile] [outputfile] [bulkoutfile] [options] General options and defaults: .TP \fB\-h\fR(elp) This help message .TP \fB\-nomenu\fR Prevent the menu interface being displayed .TP \fB\-nowarn\fR Prevent warnings about sequences being displayed .TP \fB\-silent\fR Overwrite files silently .TP \fB\-totals\fR Concatenate all genes in inputfile .TP \fB\-machine\fR Machine readable output .TP \fB\-human\fR Human readable output .TP \fB\-code\fR N Genetic code as defined under menu 3 option 5 .TP \fB\-f_type\fR N Fop/CBI codons as defined by menu 3 option 6 .TP \fB\-c_type\fR N Cai fitness values as defined by menu 3 option 7 .TP \fB\-t\fR (char) Column separator to be used in output files (comma,tab,space) .PP Codon usage indices and Amino acid indices .TP \fB\-cai\fR calculate Codon Adaptation Index (CAI) .TP \fB\-fop\fR calculate Frequency of OPtimal codons index (FOP) .TP \fB\-cbi\fR calculate Codon Bias Index (CBI) .TP \fB\-enc\fR Effective Number of Codons (ENc) .TP \fB\-gc\fR G+C content of gene (all 3 codon positions) .TP \fB\-gcs3\fR GC of synonymous codons 3rd positions .TP \fB\-sil_base\fR Base composition at synonymous third codon positions .TP \fB\-L_sym\fR Number of synonymous codons .TP \fB\-L_aa\fR Total number of synonymous and non\-synonymous codons .TP \fB\-all_indices\fR All the above indices .TP \fB\-aro\fR Calculate aromaticity of protein .TP \fB\-hyd\fR Calculate hydropathicity of protein .TP \fB\-cai_file\fR {file} User input file of CAI values .TP \fB\-cbi_file\fR {file} User input file of CBI values .TP \fB\-fop_file\fR {file} User input file of Fop values .PP Correspondence analysis (COA) options .TP \fB\-coa_cu\fR COA of codon usage frequencies .TP \fB\-coa_rscu\fR COA of Relative Synonymous Codon Usage .TP \fB\-coa_aa\fR COA of amino acid usage frequencies .TP \fB\-coa_expert\fR Generate detailed(expert) statistics on COA .TP \fB\-coa_axes\fR N Select number of axis to record .TP \fB\-coa_num\fR N Select number of genes to use to identify optimal codons values can be whole numbers or a percentage (5 or 10%) .PP Bulk output options | only one can be selected per analysis .TP \fB\-aau\fR Amino Acid Usage (AAU) .TP \fB\-raau\fR Relative Amino Acid Usage (RAAU) .TP \fB\-cu\fR Codon Usage (CU) (default) .TP \fB\-cutab\fR Tabulation of codon usage .TP \fB\-cutot\fR Tabulation of dataset's codon usage .TP \fB\-rscu\fR Relative Synonymous Codon Usage (RSCU) .TP \fB\-fasta\fR fasta format .TP \fB\-tidy\fR fasta format .TP \fB\-reader\fR Reader format (codons are separated by spaces) .TP \fB\-transl\fR Conceptual translation of DNA to amino acid .TP \fB\-base\fR Detailed report of codon G+C composition .TP \fB\-dinuc\fR Dinucleotide usage of the three codon pos. .TP \fB\-noblk\fR No bulk output to be written to file .PP Where {file} represents an input filename, and N an integer value Controlled exit <>