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Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" bp_search2gff \- turn a SearchIO report into GFF .SH "SYNOPSIS" .IX Header "SYNOPSIS" Usage: .PP .Vb 1 \& bp_search2gff [\-o outputfile] [\-f reportformat] [\-i inputfilename] OR file1 file2 .. .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" This script will turn a SearchIO report (\s-1BLAST, FASTP, SSEARCH, AXT, WABA\s0) into \s-1GFF.\s0 .PP The options are: .PP .Vb 10 \& \-i infilename \- (optional) inputfilename, will read \& either ARGV files or from STDIN \& \-o filename \- the output filename [default STDOUT] \& \-f format \- search result format (blast, fasta,waba,axt) \& (ssearch is fasta format). default is blast. \& \-t/\-\-type seqtype \- if you want to see query or hit information \& in the GFF report \& \-s/\-\-source \- specify the source (will be algorithm name \& otherwise like BLASTN) \& \-\-method \- the method tag (primary_tag) of the features \& (default is similarity) \& \-\-scorefunc \- a string or a file that when parsed evaluates \& to a closure which will be passed a feature \& object and that returns the score to be printed \& \-\-locfunc \- a string or a file that when parsed evaluates \& to a closure which will be passed two \& features, query and hit, and returns the \& location (Bio::LocationI compliant) for the \& GFF3 feature created for each HSP; the closure \& may use the clone_loc() and create_loc() \& functions for convenience, see their PODs \& \-\-onehsp \- only print the first HSP feature for each hit \& \-p/\-\-parent \- the parent to which HSP features should refer \& if not the name of the hit or query (depending \& on \-\-type) \& \-\-target/\-\-notarget \- whether to always add the Target tag or not \& \-h \- this help menu \& \-\-version \- GFF version to use (put a 3 here to use gff 3) \& \-\-component \- generate GFF component fields (chromosome) \& \-m/\-\-match \- generate a \*(Aqmatch\*(Aq line which is a container \& of all the similarity HSPs \& \-\-addid \- add ID tag in the absence of \-\-match \& \-c/\-\-cutoff \- specify an evalue cutoff .Ve .PP Additionally specify the filenames you want to process on the command-line. If no files are specified then \s-1STDIN\s0 input is assumed. You specify this by doing: bp_search2gff < file1 file2 file3 .SH "AUTHOR" .IX Header "AUTHOR" Jason Stajich, jason-at-bioperl-dot-org .SH "Contributors" .IX Header "Contributors" Hilmar Lapp, hlapp-at-gmx-dot-net .SS "clone_loc" .IX Subsection "clone_loc" Title : clone_loc Usage : my \f(CW$l\fR = clone_loc($feature\->location); Function: Helper function to simplify the task of cloning locations for \-\-locfunc closures. .PP .Vb 6 \& Presently simply implemented using Storable::dclone(). \&Example : \&Returns : A L object of the same type and with the \& same properties as the argument, but physically different. \& All structured properties will be cloned as well. \&Args : A L compliant object .Ve .SS "create_loc" .IX Subsection "create_loc" Title : create_loc Usage : my \f(CW$l\fR = create_loc(\*(L"10..12\*(R"); Function: Helper function to simplify the task of creating locations for \-\-locfunc closures. Creates a location from a feature\- table formatted string. .PP Example : Returns : A Bio::LocationI object representing the location given as formatted string. Args : A GenBank feature-table formatted string.