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TRADIS_ESSENTIALITY(1) |
User Commands |
TRADIS_ESSENTIALITY(1) |
NAME¶
tradis_essentiality - bio-tradis: produces calls of gene essentiality using an
adaptation of the method described in Langridge et al.
SYNOPSIS¶
tradis_essentiality data.tab
DESCRIPTION¶
Produces calls of gene essentiality using an adaptation of the method described
in Langridge et al. Genome Research 2009 and Barquist et al. NAR 2013. A loess
curve is fit to the distribution of insertion indices, and used to identify
the minima between the 'essential' and 'non-essential' distributions. These
distributions are then used to fit gamma distributions, which are then used to
calculate log-odds ratios, which are used to determine an insertion-index
threshold for gene essentiality. Note that this analysis requires a saturated
mutant library, and is not suitable for the analysis of data sets with low
insertion density. The script produces a number of diagnostic plots which can
be used to verify that this condition has been met.
AUTHOR¶
This manpage was written by Andreas Tille for the Debian distribution and can be
used for any other usage of the program.