.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.12. .TH SHOVILL "1" "June 2020" "shovill 1.1.0" "User Commands" .SH NAME shovill \- Assemble bacterial isolate genomes from Illumina paired-end reads .SH DESCRIPTION SYNOPSIS .IP De novo assembly pipeline for Illumina paired reads .PP USAGE .IP shovill [options] \fB\-\-outdir\fR DIR \fB\-\-R1\fR R1.fq.gz \fB\-\-R2\fR R2.fq.gz .PP GENERAL .TP \fB\-\-help\fR This help .TP \fB\-\-version\fR Print version and exit .TP \fB\-\-check\fR Check dependencies are installed .PP INPUT .TP \fB\-\-R1\fR XXX Read 1 FASTQ (default: '') .TP \fB\-\-R2\fR XXX Read 2 FASTQ (default: '') .TP \fB\-\-depth\fR N Sub\-sample \fB\-\-R1\fR/\-\-R2 to this depth. Disable with \fB\-\-depth\fR 0 (default: 150) .TP \fB\-\-gsize\fR XXX Estimated genome size eg. 3.2M (default: '') .PP OUTPUT .TP \fB\-\-outdir\fR XXX Output folder (default: '') .TP \fB\-\-force\fR Force overwrite of existing output folder (default: OFF) .TP \fB\-\-minlen\fR N Minimum contig length <0=AUTO> (default: 0) .TP \fB\-\-mincov\fR n.nn Minimum contig coverage <0=AUTO> (default: 2) .TP \fB\-\-namefmt\fR XXX Format of contig FASTA IDs in 'printf' style (default: 'contig%05d') .TP \fB\-\-keepfiles\fR Keep intermediate files (default: OFF) .PP RESOURCES .TP \fB\-\-tmpdir\fR XXX Fast temporary directory (default: '') .TP \fB\-\-cpus\fR N Number of CPUs to use (0=ALL) (default: 8) .TP \fB\-\-ram\fR n.nn Try to keep RAM usage below this many GB (default: 7.54) .PP ASSEMBLER .HP \fB\-\-assembler\fR XXX Assembler: velvet spades skesa megahit (default: 'spades') .TP \fB\-\-opts\fR XXX Extra assembler options in quotes eg. spades: '\-\-sc' (default: '') .TP \fB\-\-kmers\fR XXX K\-mers to use (default: '') .PP MODULES .TP \fB\-\-trim\fR Enable adaptor trimming (default: OFF) .TP \fB\-\-noreadcorr\fR Disable read error correction (default: OFF) .TP \fB\-\-nostitch\fR Disable read stitching (default: OFF) .TP \fB\-\-nocorr\fR Disable post\-assembly correction (default: OFF) .PP HOMEPAGE .IP https://github.com/tseemann/shovill \- Torsten Seemann .SH AUTHOR This manpage was written by Nilesh Patra for the Debian distribution and can be used for any other usage of the program.