.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.15. .TH PAIRSEQ.PY "1" "May 2020" "PairSeq.py 0.6.0" "User Commands" .SH NAME PairSeq.py \- Sorts and matches sequence records with matching coordinates across files .SH DESCRIPTION usage: PairSeq.py [\-\-version] [\-h] \fB\-1\fR SEQ_FILES_1 [SEQ_FILES_1 ...] \fB\-2\fR .IP SEQ_FILES_2 [SEQ_FILES_2 ...] [\-\-outdir OUT_DIR] [\-\-outname OUT_NAME] [\-\-failed] [\-\-fasta] [\-\-delim DELIMITER DELIMITER DELIMITER] [\-\-1f FIELDS_1 [FIELDS_1 ...]] [\-\-2f FIELDS_2 [FIELDS_2 ...]] [\-\-act {min,max,sum,set,cat}] [\-\-coord {illumina,solexa,sra,454,presto}] .PP Sorts and matches sequence records with matching coordinates across files .SS "help:" .TP \fB\-\-version\fR show program's version number and exit .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .SS "standard arguments:" .TP \fB\-1\fR SEQ_FILES_1 [SEQ_FILES_1 ...] An ordered list of FASTA/FASTQ files containing head/primary sequences. (default: None) .TP \fB\-2\fR SEQ_FILES_2 [SEQ_FILES_2 ...] An ordered list of FASTA/FASTQ files containing tail/secondary sequences. (default: None) .TP \fB\-\-outdir\fR OUT_DIR Specify to changes the output directory to the location specified. The input file directory is used if this is not specified. (default: None) .TP \fB\-\-outname\fR OUT_NAME Changes the prefix of the successfully processed output file to the string specified. May not be specified with multiple input files. (default: None) .TP \fB\-\-failed\fR If specified create files containing records that fail processing. (default: False) .TP \fB\-\-fasta\fR Specify to force output as FASTA rather than FASTQ. (default: None) .TP \fB\-\-delim\fR DELIMITER DELIMITER DELIMITER A list of the three delimiters that separate annotation blocks, field names and values, and values within a field, respectively. (default: ('|', '=', \&',')) .SS "pairing arguments:" .TP \fB\-\-1f\fR FIELDS_1 [FIELDS_1 ...] The annotation fields to copy from file 1 records into file 2 records. If a copied annotation already exists in a file 2 record, then the annotations copied from file 1 will be added to the front of the existing annotation. (default: None) .TP \fB\-\-2f\fR FIELDS_2 [FIELDS_2 ...] The annotation fields to copy from file 2 records into file 1 records. If a copied annotation already exists in a file 1 record, then the annotations copied from file 2 will be added to the end of the existing annotation. (default: None) .TP \fB\-\-act\fR {min,max,sum,set,cat} The collapse actions to take on all fields copied between files to combine duplicate fields into a single value. The actions "min", "max", "sum" perform the corresponding mathematical operation on numeric annotations. The action "set" collapses annotations into a comma delimited list of unique values. The action "cat" concatenates the values together into a single string. Only applies if the field already exists in the header before being copying from the other file. (default: None) .TP \fB\-\-coord\fR {illumina,solexa,sra,454,presto} The format of the sequence identifier which defines shared coordinate information across mate pairs. (default: presto) .SS "output files:" .IP pair\-pass .IP successfully paired reads with modified annotations. .IP pair\-fail .IP raw reads that could not be assigned to a mate\-pair. .SS "output annotation fields:" .IP .IP annotation fields specified by the \fB\-\-1f\fR or \fB\-\-2f\fR arguments. .SH AUTHOR This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.