.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.8. .TH MINIA "1" "January 2019" "minia 3.2.0" "User Commands" .SH NAME minia \- short-read biological sequence assembler .SH DESCRIPTION Specifiy \fB\-in\fR .PP [minia options] .IP [assembly options] .TP \fB\-in\fR (1 arg) : input reads (fasta/fastq/compressed) or hdf5 file [default ''] .TP \fB\-keep\-isolated\fR (0 arg) : keep short (<= max(2k, 150 bp)) isolated output sequences .TP \fB\-traversal\fR (1 arg) : traversal type ('contig', 'unitig') [default 'contig'] .TP \fB\-fasta\-line\fR (1 arg) : number of nucleotides per line in fasta output (0 means one line) [default '0'] .IP [graph simplifications options] .TP \fB\-no\-bulge\-removal\fR (0 arg) : ask to not perform bulge removal .TP \fB\-no\-tip\-removal\fR (0 arg) : ask to not perform tip removal .TP \fB\-no\-ec\-removal\fR (0 arg) : ask to not perform erroneous connection removal .TP \fB\-tip\-len\-topo\-kmult\fR (1 arg) : remove all tips of length <= k * X bp [default '2.500000'] .TP \fB\-tip\-len\-rctc\-kmult\fR (1 arg) : remove tips that pass coverage criteria, of length <= k * X bp [default '10.000000'] .TP \fB\-tip\-rctc\-cutoff\fR (1 arg) : tip relative coverage coefficient: mean coverage of neighbors > X * tip coverage [default '2.000000'] .TP \fB\-bulge\-len\-kmult\fR (1 arg) : bulges shorter than k*X bp are candidate to be removed [default '3.000000'] .TP \fB\-bulge\-len\-kadd\fR (1 arg) : bulges shorter than k+X bp are candidate to be removed [default '100'] .TP \fB\-bulge\-altpath\-kadd\fR (1 arg) : explore up to k+X nodes to find alternative path [default '50'] .TP \fB\-bulge\-altpath\-covmult\fR (1 arg) : bulges of coverage <= X*cov_altpath will be removed [default '1'] .TP \fB\-ec\-len\-kmult\fR (1 arg) : EC shorter than k*X bp are candidates to be removed [default '9.000000'] .TP \fB\-ec\-rctc\-cutoff\fR (1 arg) : EC relative coverage coefficient (similar in spirit as tip) [default '4.000000'] .TP \fB\-no\-mphf\fR (0 arg) : don't construct the MPHF .IP [kmer count options] .TP \fB\-kmer\-size\fR (1 arg) : size of a kmer [default '31'] .TP \fB\-abundance\-min\fR (1 arg) : min abundance threshold for solid kmers [default '2'] .TP \fB\-abundance\-max\fR (1 arg) : max abundance threshold for solid kmers [default '2147483647'] .TP \fB\-abundance\-min\-threshold\fR (1 arg) : min abundance hard threshold (only used when min abundance is "auto") [default '2'] .TP \fB\-histo\-max\fR (1 arg) : max number of values in kmers histogram [default '10000'] .TP \fB\-solidity\-kind\fR (1 arg) : way to compute counts of several files (sum, min, max, one, all, custom) [default 'sum'] .TP \fB\-solidity\-custom\fR (1 arg) : when solidity\-kind is custom, specifies list of files where kmer must be present [default ''] .TP \fB\-max\-memory\fR (1 arg) : max memory (in MBytes) [default '5000'] .TP \fB\-max\-disk\fR (1 arg) : max disk (in MBytes) [default '0'] .TP \fB\-solid\-kmers\-out\fR (1 arg) : output file for solid kmers (only when constructing a graph) [default ''] .TP \fB\-out\fR (1 arg) : output file [default ''] .TP \fB\-out\-dir\fR (1 arg) : output directory [default '.'] .TP \fB\-out\-tmp\fR (1 arg) : output directory for temporary files [default '.'] .TP \fB\-out\-compress\fR (1 arg) : h5 compression level (0:none, 9:best) [default '0'] .TP \fB\-storage\-type\fR (1 arg) : storage type of kmer counts ('hdf5' or 'file') [default 'hdf5'] .TP \fB\-histo2D\fR (1 arg) : compute the 2D histogram (with first file = genome, remaining files = reads) [default '0'] .TP \fB\-histo\fR (1 arg) : output the kmer abundance histogram [default '0'] .IP [kmer count, advanced performance tweaks options] .TP \fB\-minimizer\-type\fR (1 arg) : minimizer type (0=lexi, 1=freq) [default '0'] .TP \fB\-minimizer\-size\fR (1 arg) : size of a minimizer [default '10'] .TP \fB\-repartition\-type\fR (1 arg) : minimizer repartition (0=unordered, 1=ordered) [default '0'] .IP [bloom options] .TP \fB\-bloom\fR (1 arg) : bloom type ('basic', 'cache', 'neighbor') [default 'neighbor'] .TP \fB\-debloom\fR (1 arg) : debloom type ('none', 'original' or 'cascading') [default 'cascading'] .TP \fB\-debloom\-impl\fR (1 arg) : debloom impl ('basic', 'minimizer') [default 'minimizer'] .IP [branching options] .TP \fB\-branching\-nodes\fR (1 arg) : branching type ('none' or 'stored') [default 'stored'] .TP \fB\-topology\-stats\fR (1 arg) : topological information level (0 for none) [default '0'] .IP [general options] .TP \fB\-config\-only\fR (0 arg) : dump config only .TP \fB\-nb\-cores\fR (1 arg) : number of cores [default '0'] .TP \fB\-verbose\fR (1 arg) : verbosity level [default '1'] .TP \fB\-integer\-precision\fR (1 arg) : integers precision (0 for optimized value) [default '0'] .TP [debug options] .TP \fB\-redo\-bcalm\fR (0 arg) : debug function, redo the bcalm algo .TP \fB\-skip\-bcalm\fR (0 arg) : same, but skip bcalm .TP \fB\-redo\-bglue\fR (0 arg) : same, but redo bglue .TP \fB\-skip\-bglue\fR (0 arg) : same, but skip bglue .TP \fB\-redo\-links\fR (0 arg) : same, but redo links .TP \fB\-skip\-links\fR (0 arg) : same, but skip links .TP \fB\-nb\-glue\-partitions\fR (1 arg) : number of glue partitions (automatically calculated by default) [default '0'] .SH AUTHOR This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.