.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.16. .TH MACS2_BDGPEAKCALL "1" "December 2020" "macs2 bdgpeakcall 2.2.7.1" "User Commands" .SH NAME macs2_bdgpeakcall \- Model\-based Analysis for ChIP\-Sequencing .SH DESCRIPTION usage: macs2 bdgpeakcall [\-h] \fB\-i\fR IFILE [\-c CUTOFF] [\-l MINLEN] [\-g MAXGAP] .TP [\-\-cutoff\-analysis] [\-\-no\-trackline] [\-\-outdir OUTDIR] (\fB\-o\fR OFILE | \fB\-\-o\-prefix\fR OPREFIX) .SS "optional arguments:" .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-i\fR IFILE, \fB\-\-ifile\fR IFILE MACS score in bedGraph. REQUIRED .TP \fB\-c\fR CUTOFF, \fB\-\-cutoff\fR CUTOFF Cutoff depends on which method you used for score track. If the file contains pvalue scores from MACS2, score 5 means pvalue 1e\-5. DEFAULT: 5 .TP \fB\-l\fR MINLEN, \fB\-\-min\-length\fR MINLEN minimum length of peak, better to set it as d value. DEFAULT: 200 .TP \fB\-g\fR MAXGAP, \fB\-\-max\-gap\fR MAXGAP maximum gap between significant points in a peak, better to set it as tag size. DEFAULT: 30 .TP \fB\-\-cutoff\-analysis\fR While set, bdgpeakcall will analyze number or total length of peaks that can be called by different cutoff then output a summary table to help user decide a better cutoff. Note, minlen and maxgap may affect the results. DEFAULT: False .TP \fB\-\-no\-trackline\fR Tells MACS not to include trackline with bedGraph files. The trackline is required by UCSC. .TP \fB\-\-outdir\fR OUTDIR If specified all output files will be written to that directory. Default: the current working directory .TP \fB\-o\fR OFILE, \fB\-\-ofile\fR OFILE Output file name. Mutually exclusive with \fB\-\-o\-prefix\fR. .TP \fB\-\-o\-prefix\fR OPREFIX Output file prefix. Mutually exclusive with \fB\-o\fR/\-\-ofile.