.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.16. .TH MACS2_BDGBROADCALL "1" "December 2020" "macs2 bdgbroadcall 2.2.7.1" "User Commands" .SH NAME macs2_bdgbroadcall \- Model\-based Analysis for ChIP\-Sequencing .SH DESCRIPTION usage: macs2 bdgbroadcall [\-h] \fB\-i\fR IFILE [\-c CUTOFFPEAK] [\-C CUTOFFLINK] .TP [\-l MINLEN] [\-g LVL1MAXGAP] [\-G LVL2MAXGAP] [\-\-no\-trackline] [\-\-outdir OUTDIR] (\fB\-o\fR OFILE | \fB\-\-o\-prefix\fR OPREFIX) .SS "optional arguments:" .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-i\fR IFILE, \fB\-\-ifile\fR IFILE MACS score in bedGraph. REQUIRED .TP \fB\-c\fR CUTOFFPEAK, \fB\-\-cutoff\-peak\fR CUTOFFPEAK Cutoff for peaks depending on which method you used for score track. If the file contains qvalue scores from MACS2, score 2 means qvalue 0.01. DEFAULT: 2 .TP \fB\-C\fR CUTOFFLINK, \fB\-\-cutoff\-link\fR CUTOFFLINK Cutoff for linking regions/low abundance regions depending on which method you used for score track. If the file contains qvalue scores from MACS2, score 1 means qvalue 0.1, and score 0.3 means qvalue 0.5. DEFAULT: 1 .TP \fB\-l\fR MINLEN, \fB\-\-min\-length\fR MINLEN minimum length of peak, better to set it as d value. DEFAULT: 200 .TP \fB\-g\fR LVL1MAXGAP, \fB\-\-lvl1\-max\-gap\fR LVL1MAXGAP maximum gap between significant peaks, better to set it as tag size. DEFAULT: 30 .TP \fB\-G\fR LVL2MAXGAP, \fB\-\-lvl2\-max\-gap\fR LVL2MAXGAP maximum linking between significant peaks, better to set it as 4 times of d value. DEFAULT: 800 .TP \fB\-\-no\-trackline\fR Tells MACS not to include trackline with bedGraph files. The trackline is required by UCSC. .TP \fB\-\-outdir\fR OUTDIR If specified all output files will be written to that directory. Default: the current working directory .TP \fB\-o\fR OFILE, \fB\-\-ofile\fR OFILE Output file name. Mutually exclusive with \fB\-\-o\-prefix\fR. .TP \fB\-\-o\-prefix\fR OPREFIX Output file prefix. Mutually exclusive with \fB\-o\fR/\-\-ofile.