.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.8. .TH KMA "1" "February 2019" "kma 1.1.7" "User Commands" .SH NAME kma \- mapps raw reads to a template database .SH DESCRIPTION mapps raw reads to a template database. .SH OPTIONS .TP \fB\-o\fR Output file None REQUIRED .TP \fB\-t_db\fR Template DB None REQUIRED .TP \fB\-i\fR Input file name(s) STDIN .TP \fB\-ipe\fR Input paired end file name(s) .TP \fB\-int\fR Input interleaved file name(s) .TP \fB\-k\fR Kmersize DB defined .TP \fB\-e\fR evalue 0.05 .TP \fB\-ConClave\fR ConClave version 1 .TP \fB\-mem_mode\fR Use kmers to choose best template, and save memory False .TP \fB\-ex_mode\fR Searh kmers exhaustively False .TP \fB\-ef\fR Print additional features False .TP \fB\-vcf\fR Make vcf file, 2 to apply FT False/0 .TP \fB\-deCon\fR Remove contamination False .TP \fB\-dense\fR Do not allow insertions in assembly False .TP \fB\-ref_fsa\fR Consensus sequnce will have "n" instead of gaps False .TP \fB\-matrix\fR Print assembly matrix False .TP \fB\-a\fR Print all best mappings False .TP \fB\-mp\fR Minimum phred score 30 .TP \fB\-5p\fR Cut a constant number of nucleotides from the 5 prime. 0 .TP \fB\-Sparse\fR Only count kmers False .TP \fB\-Mt1\fR Map only to "num" template. 0 / False .TP \fB\-ID\fR Minimum ID 1.0% .TP \fB\-ss\fR Sparse sorting (q,c,d) q .TP \fB\-pm\fR Pairing method (p,u,f) u .TP \fB\-fpm\fR Fine Pairing method (p,u,f) u .TP \fB\-apm\fR Sets both pm and fpm u .TP \fB\-shm\fR Use shared DB made by kma_shm 0 (lvl) .TP \fB\-swap\fR Swap DB to disk 0 (lvl) .TP \fB\-1t1\fR Skip HMM False .TP \fB\-ck\fR Count kmers instead of pseudo alignment False .TP \fB\-ca\fR Make circular alignments False .TP \fB\-boot\fR Bootstrap sequence False .TP \fB\-bc\fR Base calls should be significantly overrepresented. [True] .TP \fB\-bc90\fR Base calls should be both significantly overrepresented, and have 90% agreement. False .TP \fB\-bcNano\fR Call bases at suspicious deletions, made for nanopore. False .TP \fB\-and\fR Both mrs and p_value thresholds has to reached to in order to report a template hit. or .TP \fB\-mq\fR Minimum mapping quality 0 .TP \fB\-mrs\fR Minimum alignment score, normalized to alignment length 0.50 .TP \fB\-reward\fR Score for match 1 .TP \fB\-penalty\fR Penalty for mismatch \fB\-2\fR .TP \fB\-gapopen\fR Penalty for gap opening \fB\-3\fR .TP \fB\-gapextend\fR Penalty for gap extension \fB\-1\fR .TP \fB\-per\fR Reward for pairing reads 7 .TP \fB\-cge\fR Set CGE penalties and rewards False .TP \fB\-t\fR Number of threads 1 .TP \fB\-v\fR Version .TP \fB\-h\fR Shows this help message .SH AUTHOR This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.