.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.13. .TH TO_FASTA.PY "1" "March 2020" "to_fasta.py 1.1" "User Commands" .SH NAME to_fasta.py \- Remove adapters and collapse reads from FASTQ to FASTA .SH DESCRIPTION usage: to_fasta.py [\-h] [\-3 SEQ] [\-5 SEQ] [\-\-trim\-5p BP] [\-\-trim\-3p BP] .IP [\-\-seed\-5p BP] [\-\-seed\-3p BP] [\-m BP] [\-x BP] [\-s] [\-B] [\-a] FASTQ .PP Remove adapters and collapse reads from FASTQ to FASTA .SS "positional arguments:" .TP FASTQ including stdin or compressed file {zip,gz,tar,bz} .SS "optional arguments:" .TP \fB\-h\fR, \fB\-\-help\fR show this help message and exit .TP \fB\-3\fR SEQ 3'adapter sequence .TP \fB\-5\fR SEQ 5'adapter sequence .TP \fB\-\-trim\-5p\fR BP trim specified number of bases from 5'ends .TP \fB\-\-trim\-3p\fR BP trim specified number of bases from 3'ends .TP \fB\-\-seed\-5p\fR BP 5' adapter match length in bp (default: 7) .TP \fB\-\-seed\-3p\fR BP 3' adapter match length in bp (default: 7) .TP \fB\-m\fR BP minimum read length in bp (default: 16) .TP \fB\-x\fR BP maximum read length in bp (default: 36) .TP \fB\-s\fR sensitive adapter search with 1 mismatch (default: off) .TP \fB\-B\fR only print the reads with both 5' and 3' adapter matches .TP \fB\-a\fR print all reads with and without adapter matches if the reads are in the range specified with '\-m' and '\-x' .SH AUTHOR This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.