.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.48.2. .TH BOWTIE2-BUILD-L "1" "March 2021" "bowtie2-build-l 2.4.2" "User Commands" .SH NAME bowtie2-build-l \- building a colorspace index for bowtie2 .SH SYNOPSIS .B bowtie2-build-l [\fI\,options\/\fR]\fI\,* \/\fR .SH DESCRIPTION .TP Bowtie 2 version 2.4.2 by Ben Langmead (langmea@cs.jhu.edu, www.cs.jhu.edu/~langmea) .TP reference_in comma\-separated list of files with ref sequences .TP bt2_index_base write bt2l data to files with this dir/basename .PP *** Bowtie 2 indexes work only with v2 (not v1). Likewise for v1 indexes. *** .SH OPTIONS \fB\-f\fR reference files are Fasta (default) .TP \fB\-c\fR reference sequences given on cmd line (as ) .TP \fB\-a\fR/\-\-noauto disable automatic \fB\-p\fR/\-\-bmax/\-\-dcv memory\-fitting .TP \fB\-p\fR/\-\-packed use packed strings internally; slower, less memory .TP \fB\-\-bmax\fR max bucket sz for blockwise suffix\-array builder .TP \fB\-\-bmaxdivn\fR max bucket sz as divisor of ref len (default: 4) .TP \fB\-\-dcv\fR diff\-cover period for blockwise (default: 1024) .TP \fB\-\-nodc\fR disable diff\-cover (algorithm becomes quadratic) .TP \fB\-r\fR/\-\-noref don't build .3/.4 index files .TP \fB\-3\fR/\-\-justref just build .3/.4 index files .TP \fB\-o\fR/\-\-offrate SA is sampled every 2^ BWT chars (default: 5) .TP \fB\-t\fR/\-\-ftabchars # of chars consumed in initial lookup (default: 10) .TP \fB\-\-threads\fR # of threads .TP \fB\-\-seed\fR seed for random number generator .TP \fB\-q\fR/\-\-quiet verbose output (for debugging) .TP \fB\-h\fR/\-\-help print detailed description of tool and its options .TP \fB\-\-usage\fR print this usage message .TP \fB\-\-version\fR print version information and quit .PP