.\" Automatically generated by Pod::Man 4.11 (Pod::Simple 3.35) .\" .\" Standard preamble: .\" ======================================================================== .de Sp \" Vertical space (when we can't use .PP) .if t .sp .5v .if n .sp .. .de Vb \" Begin verbatim text .ft CW .nf .ne \\$1 .. .de Ve \" End verbatim text .ft R .fi .. .\" Set up some character translations and predefined strings. \*(-- will .\" give an unbreakable dash, \*(PI will give pi, \*(L" will give a left .\" double quote, and \*(R" will give a right double quote. \*(C+ will .\" give a nicer C++. Capital omega is used to do unbreakable dashes and .\" therefore won't be available. \*(C` and \*(C' expand to `' in nroff, .\" nothing in troff, for use with C<>. .tr \(*W- .ds C+ C\v'-.1v'\h'-1p'\s-2+\h'-1p'+\s0\v'.1v'\h'-1p' .ie n \{\ . ds -- \(*W- . ds PI pi . if (\n(.H=4u)&(1m=24u) .ds -- \(*W\h'-12u'\(*W\h'-12u'-\" diablo 10 pitch . if (\n(.H=4u)&(1m=20u) .ds -- \(*W\h'-12u'\(*W\h'-8u'-\" diablo 12 pitch . ds L" "" . ds R" "" . ds C` "" . ds C' "" 'br\} .el\{\ . ds -- \|\(em\| . ds PI \(*p . ds L" `` . ds R" '' . ds C` . ds C' 'br\} .\" .\" Escape single quotes in literal strings from groff's Unicode transform. .ie \n(.g .ds Aq \(aq .el .ds Aq ' .\" .\" If the F register is >0, we'll generate index entries on stderr for .\" titles (.TH), headers (.SH), subsections (.SS), items (.Ip), and index .\" entries marked with X<> in POD. Of course, you'll have to process the .\" output yourself in some meaningful fashion. .\" .\" Avoid warning from groff about undefined register 'F'. .de IX .. .nr rF 0 .if \n(.g .if rF .nr rF 1 .if (\n(rF:(\n(.g==0)) \{\ . if \nF \{\ . de IX . tm Index:\\$1\t\\n%\t"\\$2" .. . if !\nF==2 \{\ . nr % 0 . nr F 2 . \} . \} .\} .rr rF .\" ======================================================================== .\" .IX Title "BP_SEARCH2GFF 1p" .TH BP_SEARCH2GFF 1p "2020-10-28" "perl v5.30.3" "User Contributed Perl Documentation" .\" For nroff, turn off justification. Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" bp_search2gff \- turn a SearchIO report into GFF .SH "SYNOPSIS" .IX Header "SYNOPSIS" Usage: .PP .Vb 1 \& bp_search2gff [\-o outputfile] [\-f reportformat] [\-i inputfilename] OR file1 file2 .. .Ve .SH "DESCRIPTION" .IX Header "DESCRIPTION" This script will turn a SearchIO report (\s-1BLAST, FASTP, SSEARCH, AXT, WABA\s0) into \s-1GFF.\s0 .PP The options are: .PP .Vb 10 \& \-i infilename \- (optional) inputfilename, will read \& either ARGV files or from STDIN \& \-o filename \- the output filename [default STDOUT] \& \-f format \- search result format (blast, fasta,waba,axt) \& (ssearch is fasta format). default is blast. \& \-t/\-\-type seqtype \- if you want to see query or hit information \& in the GFF report \& \-s/\-\-source \- specify the source (will be algorithm name \& otherwise like BLASTN) \& \-\-method \- the method tag (primary_tag) of the features \& (default is similarity) \& \-\-scorefunc \- a string or a file that when parsed evaluates \& to a closure which will be passed a feature \& object and that returns the score to be printed \& \-\-locfunc \- a string or a file that when parsed evaluates \& to a closure which will be passed two \& features, query and hit, and returns the \& location (Bio::LocationI compliant) for the \& GFF3 feature created for each HSP; the closure \& may use the clone_loc() and create_loc() \& functions for convenience, see their PODs \& \-\-onehsp \- only print the first HSP feature for each hit \& \-p/\-\-parent \- the parent to which HSP features should refer \& if not the name of the hit or query (depending \& on \-\-type) \& \-\-target/\-\-notarget \- whether to always add the Target tag or not \& \-h \- this help menu \& \-\-version \- GFF version to use (put a 3 here to use gff 3) \& \-\-component \- generate GFF component fields (chromosome) \& \-m/\-\-match \- generate a \*(Aqmatch\*(Aq line which is a container \& of all the similarity HSPs \& \-\-addid \- add ID tag in the absence of \-\-match \& \-c/\-\-cutoff \- specify an evalue cutoff .Ve .PP Additionally specify the filenames you want to process on the command-line. If no files are specified then \s-1STDIN\s0 input is assumed. You specify this by doing: bp_search2gff < file1 file2 file3 .SH "AUTHOR" .IX Header "AUTHOR" Jason Stajich, jason-at-bioperl-dot-org .SH "Contributors" .IX Header "Contributors" Hilmar Lapp, hlapp-at-gmx-dot-net .SS "clone_loc" .IX Subsection "clone_loc" Title : clone_loc Usage : my \f(CW$l\fR = clone_loc($feature\->location); Function: Helper function to simplify the task of cloning locations for \-\-locfunc closures. .PP .Vb 6 \& Presently simply implemented using Storable::dclone(). \&Example : \&Returns : A L object of the same type and with the \& same properties as the argument, but physically different. \& All structured properties will be cloned as well. \&Args : A L compliant object .Ve .SS "create_loc" .IX Subsection "create_loc" Title : create_loc Usage : my \f(CW$l\fR = create_loc(\*(L"10..12\*(R"); Function: Helper function to simplify the task of creating locations for \-\-locfunc closures. Creates a location from a feature\- table formatted string. .PP Example : Returns : A Bio::LocationI object representing the location given as formatted string. Args : A GenBank feature-table formatted string.