.\" Automatically generated by Pod::Man 4.11 (Pod::Simple 3.35) .\" .\" Standard preamble: .\" ======================================================================== .de Sp \" Vertical space (when we can't use .PP) .if t .sp .5v .if n .sp .. .de Vb \" Begin verbatim text .ft CW .nf .ne \\$1 .. .de Ve \" End verbatim text .ft R .fi .. .\" Set up some character translations and predefined strings. \*(-- will .\" give an unbreakable dash, \*(PI will give pi, \*(L" will give a left .\" double quote, and \*(R" will give a right double quote. \*(C+ will .\" give a nicer C++. Capital omega is used to do unbreakable dashes and .\" therefore won't be available. \*(C` and \*(C' expand to `' in nroff, .\" nothing in troff, for use with C<>. .tr \(*W- .ds C+ C\v'-.1v'\h'-1p'\s-2+\h'-1p'+\s0\v'.1v'\h'-1p' .ie n \{\ . ds -- \(*W- . ds PI pi . if (\n(.H=4u)&(1m=24u) .ds -- \(*W\h'-12u'\(*W\h'-12u'-\" diablo 10 pitch . if (\n(.H=4u)&(1m=20u) .ds -- \(*W\h'-12u'\(*W\h'-8u'-\" diablo 12 pitch . ds L" "" . ds R" "" . ds C` "" . ds C' "" 'br\} .el\{\ . ds -- \|\(em\| . ds PI \(*p . ds L" `` . ds R" '' . ds C` . ds C' 'br\} .\" .\" Escape single quotes in literal strings from groff's Unicode transform. .ie \n(.g .ds Aq \(aq .el .ds Aq ' .\" .\" If the F register is >0, we'll generate index entries on stderr for .\" titles (.TH), headers (.SH), subsections (.SS), items (.Ip), and index .\" entries marked with X<> in POD. Of course, you'll have to process the .\" output yourself in some meaningful fashion. .\" .\" Avoid warning from groff about undefined register 'F'. .de IX .. .nr rF 0 .if \n(.g .if rF .nr rF 1 .if (\n(rF:(\n(.g==0)) \{\ . if \nF \{\ . de IX . tm Index:\\$1\t\\n%\t"\\$2" .. . if !\nF==2 \{\ . nr % 0 . nr F 2 . \} . \} .\} .rr rF .\" ======================================================================== .\" .IX Title "Bio::Tradis::RemoveTags 3pm" .TH Bio::Tradis::RemoveTags 3pm "2020-01-11" "perl v5.30.0" "User Contributed Perl Documentation" .\" For nroff, turn off justification. Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" Bio::Tradis::RemoveTags \- Remove tags from seqs a fastq file .SH "VERSION" .IX Header "VERSION" version 1.4.5 .SH "SYNOPSIS" .IX Header "SYNOPSIS" Reads in a fastq file with tradis tags already attached to the start of the sequence Removes tags from the sequence and quality strings Outputs a file *.rmtag.fastq unless an out file is specified .PP .Vb 1 \& use Bio::Tradis::RemoveTags; \& \& my $pipeline = Bio::Tradis::RemoveTags\->new(fastqfile => \*(Aqabc\*(Aq, tag => \*(Aqabc\*(Aq); \& $pipeline\->remove_tags(); .Ve .SH "PARAMETERS" .IX Header "PARAMETERS" .SS "Required" .IX Subsection "Required" .IP "\(bu" 4 \&\f(CW\*(C`fastqfile\*(C'\fR \- path to/name of file to filter .IP "\(bu" 4 \&\f(CW\*(C`tag\*(C'\fR \- TraDIS tag to remove .SS "Optional" .IX Subsection "Optional" .IP "\(bu" 4 \&\f(CW\*(C`mismatch\*(C'\fR \- number of mismatches to allow when removing the tag. Default = 0 .IP "\(bu" 4 \&\f(CW\*(C`outfile\*(C'\fR \- defaults to \f(CW\*(C`file.rmtag.fastq\*(C'\fR for and input file named \f(CW\*(C`file.fastq\*(C'\fR .SH "METHODS" .IX Header "METHODS" \&\f(CW\*(C`remove_tags\*(C'\fR \- output all reads with the tags removed to \f(CW\*(C`outfile\*(C'\fR .SH "AUTHOR" .IX Header "AUTHOR" Carla Cummins .SH "COPYRIGHT AND LICENSE" .IX Header "COPYRIGHT AND LICENSE" This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. .PP This is free software, licensed under: .PP .Vb 1 \& The GNU General Public License, Version 3, June 2007 .Ve