.\" Automatically generated by Pod::Man 4.11 (Pod::Simple 3.35) .\" .\" Standard preamble: .\" ======================================================================== .de Sp \" Vertical space (when we can't use .PP) .if t .sp .5v .if n .sp .. .de Vb \" Begin verbatim text .ft CW .nf .ne \\$1 .. .de Ve \" End verbatim text .ft R .fi .. .\" Set up some character translations and predefined strings. \*(-- will .\" give an unbreakable dash, \*(PI will give pi, \*(L" will give a left .\" double quote, and \*(R" will give a right double quote. \*(C+ will .\" give a nicer C++. Capital omega is used to do unbreakable dashes and .\" therefore won't be available. \*(C` and \*(C' expand to `' in nroff, .\" nothing in troff, for use with C<>. .tr \(*W- .ds C+ C\v'-.1v'\h'-1p'\s-2+\h'-1p'+\s0\v'.1v'\h'-1p' .ie n \{\ . ds -- \(*W- . ds PI pi . if (\n(.H=4u)&(1m=24u) .ds -- \(*W\h'-12u'\(*W\h'-12u'-\" diablo 10 pitch . if (\n(.H=4u)&(1m=20u) .ds -- \(*W\h'-12u'\(*W\h'-8u'-\" diablo 12 pitch . ds L" "" . ds R" "" . ds C` "" . ds C' "" 'br\} .el\{\ . ds -- \|\(em\| . ds PI \(*p . ds L" `` . ds R" '' . ds C` . ds C' 'br\} .\" .\" Escape single quotes in literal strings from groff's Unicode transform. .ie \n(.g .ds Aq \(aq .el .ds Aq ' .\" .\" If the F register is >0, we'll generate index entries on stderr for .\" titles (.TH), headers (.SH), subsections (.SS), items (.Ip), and index .\" entries marked with X<> in POD. Of course, you'll have to process the .\" output yourself in some meaningful fashion. .\" .\" Avoid warning from groff about undefined register 'F'. .de IX .. .nr rF 0 .if \n(.g .if rF .nr rF 1 .if (\n(rF:(\n(.g==0)) \{\ . if \nF \{\ . de IX . tm Index:\\$1\t\\n%\t"\\$2" .. . if !\nF==2 \{\ . nr % 0 . nr F 2 . \} . \} .\} .rr rF .\" ======================================================================== .\" .IX Title "Bio::Tradis::Parser::Bam 3pm" .TH Bio::Tradis::Parser::Bam 3pm "2020-01-11" "perl v5.30.0" "User Contributed Perl Documentation" .\" For nroff, turn off justification. Always turn off hyphenation; it makes .\" way too many mistakes in technical documents. .if n .ad l .nh .SH "NAME" Bio::Tradis::Parser::Bam \- Very basic BAM parser. Limited functionality. .SH "VERSION" .IX Header "VERSION" version 1.4.5 .SH "SYNOPSIS" .IX Header "SYNOPSIS" Parses \s-1BAM\s0 files and gives access to basic info in them. .PP .Vb 1 \& use Bio::Tradis::Parser::Bam; \& \& my $pipeline = Bio::Tradis::Parser::Bam\->new(file => \*(Aqabc\*(Aq); \& $pipeline\->read_info; \& $pipeline\->next_read; \& $pipeline\->seq_info; \& $pipeline\->is_mapped; \& $pipeline\->is_reverse; .Ve .PP =seq_info Reads \s-1BAM\s0 header and returns a hash (keys are sequence ids, values are hash refs with keys as tags (like \s-1LN\s0 and M5)) .PP =next_read Moves _currentread to the next entry in the \s-1BAM.\s0 Returns 0 if \s-1EOF.\s0 .PP =read_info Returns info from _currentread = hash reference with field name as key. Standard fields are named as per the \s-1SAM\s0 format specification: 1 : \s-1QNAME 2 : FLAG 3 : RNAME 4 : POS 5 : MAPQ 6 : CIGAR 7 : RNEXT 8 : PNEXT 9 : TLEN 10 : SEQ 11 : QUAL\s0 Additional fields will use their tag names. Complete line is returned with key \s-1READ\s0 .PP =is_mapped Parses the flag for the current read and determines if mapped. Returns 0 or 1. .PP =is_reverse Parses the flag for the current read and determines if reverse complemented. Returns 0 or 1. .SH "AUTHOR" .IX Header "AUTHOR" Carla Cummins .SH "COPYRIGHT AND LICENSE" .IX Header "COPYRIGHT AND LICENSE" This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. .PP This is free software, licensed under: .PP .Vb 1 \& The GNU General Public License, Version 3, June 2007 .Ve