.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.16. .TH QUALITYSCORES "1" "March 2021" "qualityScores 2.0.1" "User Commands" .SH NAME qualityScores \- toolkit for processing next-gen sequencing data .SH DESCRIPTION qualityScore Version 2.0.1 .IP Retrieve Phred score for read bases .PP Usage: .IP \&./qualityScores [options] \fB\-i\fR \fB\-o\fR .PP Required arguments: .TP \fB\-i\fR Name of input file including read data. The default format is Fastq. .TP \fB\-o\fR Name of output file that is a text file including Phred scores for each read base. .PP Optional arguments: .HP \fB\-\-gzFASTQinput\fR Input file is in gzipped Fastq format. .TP \fB\-\-BAMinput\fR Input file is in BAM format. .TP \fB\-\-SAMinput\fR Input file is in SAM format. .TP \fB\-\-first\-end\fR Use only first reads in paired\-end data. Only applicable for paired\-end BAM/SAM input. .TP \fB\-\-second\-end\fR Use only second reads in paired\-end data. Only applicable for paired\-end BAM/SAM input. .HP \fB\-\-counted\-reads\fR Total number of reads to be extracted from the input .IP file. 10,000 by default. .HP \fB\-\-phred\-offset\fR <33|64> refer to subread aligner. .SH AUTHOR This manpage was written by Nilesh Patra for the Debian distribution and can be used for any other usage of the program.