.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.49.2. .TH MOSDEPTH "1" "November 2022" "mosdepth 0.3.3" "User Commands" .SH NAME mosdepth \- manual page for mosdepth 0.3.3 .SH DESCRIPTION mosdepth 0.3.3 .IP Usage: mosdepth [options] .PP Arguments: .TP outputs: `{prefix}.mosdepth.dist.txt` .TP `{prefix}.mosdepth.summary.txt` `{prefix}.per\-base.bed.gz` (unless \fB\-n\fR/\-\-no\-per\-base is specified) `{prefix}.regions.bed.gz` (if \fB\-\-by\fR is specified) `{prefix}.quantized.bed.gz` (if \fB\-\-quantize\fR is specified) `{prefix}.thresholds.bed.gz` (if \fB\-\-thresholds\fR is specified) .TP the alignment file for which to calculate depth. .PP Common Options: .TP \fB\-t\fR \fB\-\-threads\fR number of BAM decompression threads [default: 0] .TP \fB\-c\fR \fB\-\-chrom\fR chromosome to restrict depth calculation. .TP \fB\-b\fR \fB\-\-by\fR optional BED file or (integer) window\-sizes. .TP \fB\-n\fR \fB\-\-no\-per\-base\fR dont output per\-base depth. skipping this output will speed execution substantially. prefer quantized or thresholded values if possible. .TP \fB\-f\fR \fB\-\-fasta\fR fasta file for use with CRAM files [default: ]. .PP Other options: .TP \fB\-F\fR \fB\-\-flag\fR exclude reads with any of the bits in FLAG set [default: 1796] .TP \fB\-i\fR \fB\-\-include\-flag\fR only include reads with any of the bits in FLAG set. default is unset. [default: 0] .TP \fB\-x\fR \fB\-\-fast\-mode\fR dont look at internal cigar operations or correct mate overlaps (recommended for most use\-cases). .TP \fB\-q\fR \fB\-\-quantize\fR write quantized output see docs for description. .TP \fB\-Q\fR \fB\-\-mapq\fR mapping quality threshold. reads with a quality less than this value are ignored [default: 0] .TP \fB\-T\fR \fB\-\-thresholds\fR for each interval in \fB\-\-by\fR, write number of bases covered by at least threshold bases. Specify multiple integer values separated by ','. .TP \fB\-m\fR \fB\-\-use\-median\fR output median of each region (in \fB\-\-by\fR) instead of mean. .TP \fB\-R\fR \fB\-\-read\-groups\fR only calculate depth for these comma\-separated read groups IDs. .TP \fB\-h\fR \fB\-\-help\fR show help