.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.47.16. .TH FASTANI "1" "March 2021" "fastANI 1.32" "User Commands" .SH NAME fastANI \- Fast alignment-free computation of whole-genome Average Nucleotide Identity .SH DESCRIPTION \fB\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\fR fastANI is a fast alignment\-free implementation for computing whole\-genome Average Nucleotide Identity (ANI) between genomes \fB\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\fR Example usage: $ fastANI \fB\-q\fR genome1.fa \fB\-r\fR genome2.fa \fB\-o\fR output.txt $ fastANI \fB\-q\fR genome1.fa \fB\-\-rl\fR genome_list.txt \fB\-o\fR output.txt .PP Available options \fB\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\-\fR \fB\-h\fR, \fB\-\-help\fR .IP Print this help page .PP \fB\-r\fR , \fB\-\-ref\fR .IP reference genome (fasta/fastq)[.gz] .PP \fB\-\-refList\fR , \fB\-\-rl\fR .IP a file containing list of reference genome files, one genome per line .PP \fB\-q\fR , \fB\-\-query\fR .IP query genome (fasta/fastq)[.gz] .PP \fB\-\-ql\fR , \fB\-\-queryList\fR .IP a file containing list of query genome files, one genome per line .PP \fB\-k\fR , \fB\-\-kmer\fR .IP kmer size <= 16 [default : 16] .PP \fB\-t\fR , \fB\-\-threads\fR .IP thread count for parallel execution [default : 1] .PP \fB\-\-fragLen\fR .IP fragment length [default : 3,000] .PP \fB\-\-minFraction\fR .IP minimum fraction of genome that must be shared for trusting ANI. If reference and query genome size differ, smaller one among the two is considered. [default : 0.2] .PP \fB\-\-visualize\fR .IP output mappings for visualization, can be enabled for single genome to single genome comparison only [disabled by default] .PP \fB\-\-matrix\fR .IP also output ANI values as lower triangular matrix (format inspired from phylip). If enabled, you should expect an output file with .matrix extension [disabled by default] .PP \fB\-o\fR , \fB\-\-output\fR [required] .IP output file name .PP \fB\-v\fR, \fB\-\-version\fR .IP Show version .SH AUTHOR This manpage was written by Nilesh Patra for the Debian distribution and can be used for any other usage of the program.